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Validation of on-slide immunocytochemistry controls prepared from long-term preserved prefixed cell suspensions

2026-02-01Artigo científico Acesso aberto
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dc.contributor.authorRoque, Rúben Rodrigues
dc.contributor.authorSrebotnik Kirbiš, Irena
dc.contributor.authorPinheiro, Carla Sofia Castro
dc.contributor.authorMartins, Rita Sattler Fagulha
dc.contributor.authorTavares, Vanessa Sofia
dc.contributor.authorAndré, Saudade
dc.contributor.authorFélix, Ana
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblJohn Wiley and Sons Inc.
dc.date.accessioned2026-01-20T14:22:52Z
dc.date.available2026-01-20T14:22:52Z
dc.date.issued2026-02-01
dc.descriptionPublisher Copyright: © 2026 The Author(s). Cancer Cytopathology published by Wiley Periodicals LLC on behalf of American Cancer Society.
dc.description.abstractBACKGROUND: On-slide controls are essential for quality assurance in immunocytochemistry, yet their use in non-cell block cytology preparations remains a significant challenge. This study presents and evaluates a novel method for preparing on-slide controls from long-term prefixed cell suspensions, applicable to all types of cytology preparations. METHODS: A methodology for preparing on-slide controls is described in detail and comprehensively validated via three key evaluations: assessing antigen and cellular morphology stability in 17 suspensions over 29 months; conducting a retrospective review of immunocytochemistry records (2019-2024) to determine feasibility; and performing a tracer study to evaluate cell carryover risk. RESULTS: In the stability assessment, all 20 evaluated antigens and cellular morphologies remained well preserved in the stored control samples, with only transient partial loss of antigenicity observed on three of 149 slides. The method was successfully and reliably applied in 1904 immunocytochemistry tests (n = 48 primary antibodies), with a failure rate of 0.3% (95% CI, 0.1%-0.7%). The dedicated tracer study confirmed the complete absence of cell carryover or cross-contamination. CONCLUSIONS: The described method offers a robust, practical, and adaptable solution for preparing on-slide controls applicable to all cytology preparations, including previously stained and mounted slides. Implementing this approach strengthens quality control for non-cell block cytology slides, enhances diagnostic reliability, and brings cytology practice closer to the quality standards established in immunohistochemistry.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1868338
dc.identifier.doi10.1002/cncy.70072
dc.identifier.issn1934-662X
dc.identifier.otherPURE: 150633768
dc.identifier.otherPURE UUID: dde74f8e-12d6-4ba1-9074-ad35d1709c87
dc.identifier.otherScopus: 105026966415
dc.identifier.otherPubMed: 41504087
dc.identifier.otherPubMedCentral: PMC12781157
dc.identifier.otherORCID: /0000-0002-2653-2262/work/203079768
dc.identifier.urihttp://hdl.handle.net/10362/199556
dc.identifier.urlhttps://www.scopus.com/pages/publications/105026966415
dc.language.isoeng
dc.peerreviewedyes
dc.subjectcytospins
dc.subjectexternal controls
dc.subjectimmunocytochemistry
dc.subjecton‐slide controls
dc.subjectquality control
dc.subjectOncology
dc.subjectCancer Research
dc.subjectSDG 3 - Good Health and Well-being
dc.titleValidation of on-slide immunocytochemistry controls prepared from long-term preserved prefixed cell suspensionsen
dc.typejournal article
degois.publication.issue2
degois.publication.titleCancer Cytopathology
degois.publication.volume134
dspace.entity.typePublication
rcaap.rightsopenAccess

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