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Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst

2023Artigo científico Acesso aberto
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dc.contributor.authorEscórcio, Rita
dc.contributor.authorSandhu, Armaan K.
dc.contributor.authorBento, Artur
dc.contributor.authorTomé, Ana S.
dc.contributor.authorMoreira, Carlos J.S.
dc.contributor.authorBrözel, Volker S.
dc.contributor.authorSilva Pereira, Cristina
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblFrontiers Media
dc.date.accessioned2023-09-30T22:19:05Z
dc.date.available2023-09-30T22:19:05Z
dc.date.issued2023
dc.descriptionFunding Information: The authors acknowledge CERMAX, ITQB-NOVA, Oeiras, Portugal where the NMR data were acquired with equipment funded by FCT. The authors are thankful to Giovanna Martins for technical support during her BSc internship. Funding Information: Funding from the European Research Council through grant ERC CoG-647928, from the Office of Naval Research Global (ONRG, RESEARCH GRANT—N62909-23-1-2007), and from Fundação para a Ciência e Tecnologia (FCT) by Project MOSTMICRO ITQB with refs UIDB/04612/2020 and UIDP/04612/2020 and LS4FUTURE Associated Laboratory (LA/P/0087/2020). RE is grateful to FCT funding for her PhD scholarship (2021.06435.BD). Publisher Copyright: Copyright © 2023 Escórcio, Sandhu, Bento, Tomé, Moreira, Brözel and Silva Pereira.
dc.description.abstractSoybean (Glycine max) is an increasingly relevant crop due to its economic importance and also a model plant for the study of root symbiotic associations with nodule forming rhizobia. Plant polyesters mediate plant-microbe interactions with both pathogenic and beneficial microbes; suberin has been hypothesized to play a key role during the early steps of rhizobia attachment to the root. The downside is that suberin chemistry in soybean root is still scarcely studied. This study addresses this outstanding question by reporting a straightforward workflow for a speedy purification of suberin from soybean root and for its subsequent detailed chemical analysis. To purify suberin, cholinium hexanoate (an ionic liquid) was used as the catalyst. The ensuing suberin is highly esterified as observed by a precise Nuclear Magnetic Resonance quantification of each ester type, discriminating between primary and acylglycerol esters. Moreover, the composing hydrolysable monomers detected through GC-MS revealed that hexadecanoic acid is the most abundant monomer, similar to that reported before by others. Overall, this study highlights the adequacy of the ionic liquid catalyst for the isolation of suberin from soybean roots, where the polymer natural abundance is low, and builds new knowledge on the specificities of its chemistry; essential to better understand the biological roles of suberin in roots.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1341953
dc.identifier.doi10.3389/fchem.2023.1165234
dc.identifier.issn2296-2646
dc.identifier.otherPURE: 72375230
dc.identifier.otherPURE UUID: 8f1e08b0-da22-4258-b374-834cb119e032
dc.identifier.otherScopus: 85168661670
dc.identifier.urihttp://hdl.handle.net/10362/158529
dc.identifier.urlhttps://www.scopus.com/pages/publications/85168661670
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGlycine max
dc.subjectpurification of a suberin polymer
dc.subjectquantification of polymeric features
dc.subjectsoybean root
dc.subjectsuberin in planta
dc.subjectGeneral Chemistry
dc.titlePurification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalysten
dc.typejournal article
degois.publication.titleFrontiers in Chemistry
degois.publication.volume11
dspace.entity.typePublication
rcaap.rightsopenAccess

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