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Taxogenomic analysis of Pichia senei sp. nov. and new insights into hybridization events in the Pichia cactophila species complex

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dc.contributor.authorBarros, Katharina O.
dc.contributor.authorAl-Oboudi, Jassim
dc.contributor.authorFreitas, Larissa F.D.
dc.contributor.authorSousa, Francisca M.P.
dc.contributor.authorBatista, Thiago M.
dc.contributor.authorSantos, Ana Raquel O.
dc.contributor.authorMorais, Paula B.
dc.contributor.authorSampaio, José Paulo
dc.contributor.authorLachance, Marc André
dc.contributor.authorHittinger, Chris Todd
dc.contributor.authorRosa, Carlos A.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionFaculdade de Ciências e Tecnologia (FCT)
dc.contributor.pblFederation of European Microbiological Societies | Oxford University Press
dc.date.accessioned2025-09-04T22:38:19Z
dc.date.available2025-09-04T22:38:19Z
dc.date.issued2025
dc.descriptionThis work is part of the project \u201CINCT Yeasts: Biodiversity, preservation and biotechnological innovation\u201D, funded by Conselho Nacional de Desenvolvimento Cient\u00EDfico e Tecnol\u00F3gico (CNPq), Brazil, grant number 406564/2022-1. This work was also funded by CNPq process numbers 0457499/2014-1, 141586/2017-6, 313088/2020-9, and 408733/2021, Funda\u00E7\u00E3o do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, process numbers APQ-01525-14, APQ-02552-15, and APQ-03071\u201317), the Natural Science and Engineering Research Council of Canada (MAL), Research in the Hittinger Lab is funded by the National Science Foundation (DEB-2110403), USDA National Institute of Food and Agriculture (Hatch Project 7005101), in part by the DOE Great Lakes Bioenergy Research Center (DOE BER Office of Science DE\u2013SC0018409), and an H. I. Romnes Faculty Fellowship (Office of the Vice Chancellor for Research with funding from the Wisconsin Alumni Research Foundation). Publisher Copyright: © 2025 The Author(s). Published by Oxford University Press on behalf of FEMS.
dc.description.abstractThree strains of a novel yeast species were isolated from necrotic cactus tissues of Cereus saddianus and Micranthocereus dolichospermaticus and from phytotelmata of Bromelia karatas. DNA sequence analysis of the Internal Transcribed Spacer (ITS) region and D1/D2 domains of the large subunit ribosomal RNA, along with whole genome phylogenomic analysis, showed that this yeast is most closely related to Pichia insulana, Pichia cactophila, and Pichia inconspicua. The new species differs by 10-13 nucleotide substitutions from these species in D1/D2 sequences and exhibits <90% genome-wide average nucleotide identity to them. The name Pichia senei sp. nov. is proposed for the novel species, which is homothallic and produces asci with one to four hat-shaped ascospores. The holotype is CBS 16311 (MycoBank MB 858723). Taxogenomic analyses of the P. cactophila species complex, including P. senei, provide new insights about the hybridizations events that shaped this group. Pichia insulana and P. inconspicua are identified as the parental lineages that originated P. cactophila, and P. senei also appears closely related to one of the progenitors of P. inconspicua. We assess phylogeny, heterozygosity, and ploidy to explore the processes shaping diversity, showing how genomic data support yeast species delimitation and reveal complex hybridization.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1611980
dc.identifier.doi10.1093/femsyr/foaf037
dc.identifier.issn1567-1356
dc.identifier.otherPURE: 128701738
dc.identifier.otherPURE UUID: 44f4a545-dc62-4869-869f-b1b1e89800e1
dc.identifier.otherScopus: 105013594514
dc.identifier.otherPubMed: 40796109
dc.identifier.urihttp://hdl.handle.net/10362/187560
dc.identifier.urlhttps://www.scopus.com/pages/publications/105013594514
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/Concurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017%2F2018) - Financiamento Base/UIDB%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.subjectbromeliads
dc.subjectcacti
dc.subjecthybridizations
dc.subjectPichia cactophila complex
dc.subjectPichia senei sp. nov
dc.subjectMicrobiology
dc.subjectApplied Microbiology and Biotechnology
dc.titleTaxogenomic analysis of Pichia senei sp. nov. and new insights into hybridization events in the Pichia cactophila species complexen
dc.typejournal article
degois.publication.titleFEMS Yeast Research
degois.publication.volume25
dspace.entity.typePublication
oaire.awardNumberUIDP/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication38373452-5c9c-4724-8def-f03314ecce0e
relation.isProjectOfPublicationecd6da06-821d-4fff-933b-319b334dc19a
relation.isProjectOfPublication.latestForDiscoveryecd6da06-821d-4fff-933b-319b334dc19a

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