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Genetic diversity and antimicrobial resistance profiles of Staphylococcus pseudintermedius associated with skin and soft-tissue infections in companion animals in Lisbon, Portugal

2023-04-17Artigo científico Acesso aberto
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dc.contributor.authorMorais, Catarina
dc.contributor.authorCosta, Sofia Santos
dc.contributor.authorLeal, Marta
dc.contributor.authorRamos, Bárbara
dc.contributor.authorAndrade, Mariana
dc.contributor.authorFerreira, Carolina
dc.contributor.authorAbrantes, Patrícia
dc.contributor.authorPomba, Constança
dc.contributor.authorCouto, Isabel
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.pblFrontiers Research Foundation
dc.date.accessioned2023-07-06T22:20:34Z
dc.date.available2023-07-06T22:20:34Z
dc.date.issued2023-04-17
dc.descriptionFunding Information: This study was supported by Project BIOSAFE funded by FEDER through the Programa Operacional Factores de Competitividade–COMPETE, by the Fundação para a Ciência e a Tecnologia (FCT, Portugal) - Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017 and by FCT through grants UI/BD/151061/2021 (CM) and 2021.05063.BD (CF) and funds to GHTM (UID/04413/2020) and the CIISA Project (UID/CVT/00276/2020). Publisher Copyright: Copyright © 2023 Morais, Costa, Leal, Ramos, Andrade, Ferreira, Abrantes, Pomba and Couto.
dc.description.abstractStaphylococcus pseudintermedius is the main bacterial pathogen of skin and soft-tissue infections (SSTIs) in companion animals. Antimicrobial resistance in this species is a growing public health concern. This study aims to characterize a collection of S. pseudintermedius causing SSTIs in companion animals, establishing the main clonal lineages and antimicrobial resistance traits. The collection corresponded to all S. pseudintermedius (n = 155) causing SSTIs in companion animals (dogs, cats and one rabbit) collected between 2014 and 2018 at two laboratories in Lisbon, Portugal. Susceptibility patterns were established by disk diffusion for 28 antimicrobials (15 classes). For antimicrobials without clinical breakpoints available, a cut-off value (COWT) was estimated, based on the distribution of the zones of inhibition. The blaZ and mecA genes were screened for the entire collection. Other resistance genes (e.g., erm, tet, aadD, vga(C), dfrA(S1)) were searched only for those isolates showing an intermediate/resistance phenotype. For fluoroquinolone resistance, we determined the chromosomal mutations in the target genes grlA and gyrA. All the isolates were typed by PFGE following SmaI macrorestriction and isolates representative of each PFGE type were further typed by MLST. Forty-eight out of the 155 S. pseudintermedius isolates (31.0%) were methicillin-resistant (mecA +, MRSP). Multidrug-resistant (MDR) phenotypes were detected for 95.8% of the MRSP and 22.4% of the methicillin-susceptible (MSSP) isolates. Of particular concern, only 19 isolates (12.3%) were susceptible to all antimicrobials tested. In total, 43 different antimicrobial resistance profiles were detected, mostly associated with the carriage of blaZ, mecA, erm(B), aph3-IIIa, aacA-aphD, cat pC221, tet(M) and dfr(G) genes. The 155 isolates were distributed within 129 PFGE clusters, grouped by MLST in 42 clonal lineages, 25 of which correspond to new sequence types (STs). While ST71 remains the most frequent S. pseudintermedius lineage, other lineages that have been replacing ST71 in other countries were detected, including ST258, described for the first time in Portugal. This study revealed a high frequency of MRSP and MDR profiles among S. pseudintermedius associated with SSTIs in companion animals in our setting. Additionally, several clonal lineages with different resistance profiles were described, evidencing the importance of a correct diagnosis and selection of the therapy.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent1992122
dc.identifier.doi10.3389/fmicb.2023.1167834
dc.identifier.issn1664-302X
dc.identifier.otherPURE: 113884668
dc.identifier.otherPURE UUID: 5379e001-cbf7-46bf-90c7-88d08835f8e4
dc.identifier.otherWOS: 000978542400001
dc.identifier.otherScopus: 85152665952
dc.identifier.otherPubMed: 37138637
dc.identifier.otherPubMedCentral: PMC10149759
dc.identifier.otherORCID: /0000-0002-4096-2410/work/151391885
dc.identifier.otherORCID: /0000-0003-3536-2733/work/151411249
dc.identifier.otherORCID: /0000-0002-7484-2441/work/151431496
dc.identifier.urihttp://hdl.handle.net/10362/154953
dc.identifier.urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=nova_api&SrcAuth=WosAPI&KeyUT=WOS:000978542400001&DestLinkType=FullRecord&DestApp=WOS
dc.identifier.urlhttps://www.scopus.com/pages/publications/85152665952
dc.language.isoeng
dc.peerreviewedyes
dc.subjectMLST
dc.subjectSSTIs
dc.subjectStaphylococcus pseudintermedius
dc.subjectAntimicrobial resistance
dc.subjectClonal lineage
dc.subjectCompanion animals
dc.subjectPets
dc.subjectRZ Other systems of medicine
dc.subjectQR Microbiology
dc.subjectInfectious Diseases
dc.subjectSmall Animals
dc.subjectMicrobiology (medical)
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectPharmacology (medical)
dc.subjectSDG 3 - Good Health and Well-being
dc.subjectSDG 11 - Sustainable Cities and Communities
dc.subjectSDG 12 - Responsible Consumption and Production
dc.titleGenetic diversity and antimicrobial resistance profiles of Staphylococcus pseudintermedius associated with skin and soft-tissue infections in companion animals in Lisbon, Portugalen
dc.typejournal article
degois.publication.titleFrontiers in Microbiology
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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