Logo do repositório
 
Publicação

Detecting mir-155-3p through a Molecular Beacon Bead-Based Assay

2024-07-03Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorMoreira, David
dc.contributor.authorAlexandre, Daniela
dc.contributor.authorMiranda, André
dc.contributor.authorLourenço, Pedro
dc.contributor.authorBaptista, Pedro V.
dc.contributor.authorTomaz, Cândida
dc.contributor.authorLu, Yi
dc.contributor.authorCruz, Carla
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2024-10-10T22:39:13Z
dc.date.available2024-10-10T22:39:13Z
dc.date.issued2024-07-03
dc.descriptionFunding Information: This study was developed within the scope of the CICS-UBI projects 10.54499/UIDB/00709/2020 and 10.54499/UIDP/00709/2020; LA/P/0140/2020, financed by national funds through the Portuguese Foundation for Science and Technology/MCTES; “Bolsa de Investigação em Oncologia Dr. Rocha Alves do Núcleo Regional do Centro da Liga Portuguesa Contra o Cancro”, Instruct-ERIC Pilot R&D application ID 2473, and PAPILOMA ref. CENTRO-01-0145-FEDER-181235. David Moreira, André Miranda, and Daniela Alexandre acknowledge doctoral fellowship grants from FCT https://doi.org/10.54499/2023.02001.BD, https://doi.org/10.54499/2021.07695.BD and https://sciproj.ptcris.pt/80505DFA, respectively. Pedro Lourenço acknowledges a fellowship grant financed by FCT to CICS-UBI (ref. UIDP/00709/2020). Publisher Copyright: © 2024 by the authors.
dc.description.abstractLung cancer (LC) is recognized as one of the most prevalent and lethal cancers worldwide, underscoring an urgent need for innovative diagnostic and therapeutic approaches. MicroRNAs (miRNAs) have emerged as promising biomarkers for several diseases and their progression, such as LC. However, traditional methods for detecting and quantifying miRNAs, such as PCR, are time-consuming and expensive. Herein, we used a molecular beacon (MB) bead-based assay immobilized in a microfluidic device to detect miR-155-3p, which is frequently overexpressed in LC. The assay relies on the fluorescence enhancement of the MB upon binding to the target miRNA via Watson and Crick complementarity, resulting in a conformational change from a stem–loop to a linear structure, thereby bringing apart the fluorophores at each end. This assay was performed on a microfluidic platform enabling rapid and straightforward target detection. We successfully detected miR-155-3p in a saline solution, obtaining a limit of detection (LOD) of 42 nM. Furthermore, we evaluated the method’s performance in more complex biological samples, including A549 cells’ total RNA and peripheral blood mononuclear cells (PBMCs) spiked with the target miRNA. We achieved satisfactory recovery rates, especially in A549 cells’ total RNA.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent10
dc.format.extent1739296
dc.identifier.doi10.3390/molecules29133182
dc.identifier.issn1420-3049
dc.identifier.otherPURE: 99281614
dc.identifier.otherPURE UUID: 05fe393f-98ce-4e1c-99cb-845d1b790a37
dc.identifier.otherScopus: 85198476690
dc.identifier.otherWOS: 001269705500001
dc.identifier.otherPubMed: 38999134
dc.identifier.otherPubMedCentral: PMC11243622
dc.identifier.otherORCID: /0000-0001-5255-7095/work/169339058
dc.identifier.urihttp://hdl.handle.net/10362/173336
dc.identifier.urlhttps://www.scopus.com/pages/publications/85198476690
dc.language.isoeng
dc.peerreviewedyes
dc.subjectlung cancer
dc.subjectmicrofluidics
dc.subjectmir-155-3p
dc.subjectmolecular beacon
dc.subjectAnalytical Chemistry
dc.subjectChemistry (miscellaneous)
dc.subjectMolecular Medicine
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleDetecting mir-155-3p through a Molecular Beacon Bead-Based Assayen
dc.typejournal article
degois.publication.issue13
degois.publication.titleMolecules
degois.publication.volume29
dspace.entity.typePublication
rcaap.rightsopenAccess

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Detecting_mir-155-3p_through_a_Molecular_Beacon.pdf
Tamanho:
1.66 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

FCT: DCV - Artigos em revista internacional com arbitragem científica