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The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells

2022-02Artigo científico Acesso aberto
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dc.contributor.authorBugalhão, Joana N.
dc.contributor.authorLuís, Maria P.
dc.contributor.authorPereira, Inês Serrano
dc.contributor.authorda Cunha, Maria
dc.contributor.authorPais, Sara V.
dc.contributor.authorMota, Luís Jaime
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2022-03-11T23:23:22Z
dc.date.available2022-03-11T23:23:22Z
dc.date.issued2022-02
dc.descriptionPTDC/BIA-MIC/28503/2017 PTDC/IMI-MIC/1300/2014 UIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 PD/BD/128214/2016 PD/BD/52210/2013 PD/00133/2012 SFRH/BD/129756/2017 SFRH/BD/144284/2019
dc.description.abstractChlamydia trachomatis causes genital and ocular infections in humans. This bacterial pathogen multiplies exclusively within host cells in a characteristic vacuole (inclusion) and delivers proteins such as inclusion membrane proteins (Incs) into the host cell. Here, we identified CT006 as a novel C. trachomatis protein that when expressed ectopically eukaryotic cells can associate with lipid droplets (LDs). A screen using Saccharomyces cerevisiae identified two Incs causing vacuolar protein sorting defects and seven Incs showing tropism for eukaryotic organelles. Ectopic expression in yeast and mammalian cells of genes encoding different fragments of CT006 revealed tropism for the endoplasmic reticulum and LDs. We identified a LD-targeting region within the first 88 amino acid residues of CT006, and positively charged residues important for this targeting. Comparing with the parental wild-type strain, cells infected by a newly generated C. trachomatis strain overproducing CT006 with a double hemagglutinin tag showed a slight increase in the area occupied by LDs within the inclusion region. However, we could not correlate this effect with the LD-targeting regions within CT006. We further showed that both the amino and carboxy-terminal regions of CT006, flanking the Inc-characteristic bilobed hydrophobic domain, are exposed to the host cell cytosol during C. trachomatis infection, supporting their availability to interact with host cell targets. Altogether, our data suggest that CT006 might participate in the interaction of LDs with C. trachomatis inclusions.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent3456964
dc.identifier.doi10.1371/journal.pone.0264292
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 42072364
dc.identifier.otherPURE UUID: e7834e10-06f6-4df2-b1bf-abbf382110e6
dc.identifier.otherPubMed: 35192658
dc.identifier.otherPubMedCentral: PMC8863265
dc.identifier.otherScopus: 85125156570
dc.identifier.otherORCID: /0000-0002-5592-8397/work/109728136
dc.identifier.otherWOS: 000812552500047
dc.identifier.urihttp://hdl.handle.net/10362/134356
dc.identifier.urlhttps://www.scopus.com/pages/publications/85125156570
dc.language.isoeng
dc.peerreviewedyes
dc.titleThe Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cellsen
dc.typejournal article
degois.publication.firstPage
degois.publication.issue2
degois.publication.lastPage
degois.publication.titlePLoS ONE
degois.publication.volume17
dspace.entity.typePublication
rcaap.rightsopenAccess

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