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Comparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA tests

2019-12-01Artigo científico Acesso aberto
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dc.contributor.authorMoreno, Yorleydy Ruiz
dc.contributor.authorDonato, Silvia Tavares
dc.contributor.authorNogueira, Fátima
dc.contributor.authorSilva, Marcelo Sousa
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-05-01T22:52:45Z
dc.date.available2021-05-01T22:52:45Z
dc.date.issued2019-12-01
dc.description.abstractEarly diagnosis of malaria reduces disease, prevents deaths, and contributes to decreased malaria transmission. The use of specific and sensitive antigens in the execution of serological diagnostics may have an impact on the transmission of the disease. However, many individuals cannot be easily diagnosed by serological tests due to low levels of antibodies in the serum. Using two different Enzyme-Linked Immunosorbent Assay (ELISA) tests (a commercial and an in-house ELISA), a total of 365 serum samples from individuals with a clinical history of malaria were analyzed. From the serum samples analyzed, 192 (53%) samples from the commercial ELISA and 219 (60%) samples from the in-house ELISA presented positive serological reactivity to malaria. The concordance of the samples tested (n = 365) between both ELISAs was of 67% (n = 242), and with the negative control was 100% (n = 17). We demonstrated that the in-house ELISA showed high antigenic reactivity to Plasmodium falciparum antigens when compared with the commercial ELISA. The degree of concordance of both ELISAs suggested the possibility of existence of other P. falciparum antigens present in the crude extract of P. falciparum that are important in the serological response during malaria infection.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent3019343
dc.identifier.doi10.3390/diagnostics9040168
dc.identifier.issn2075-4418
dc.identifier.otherPURE: 26649719
dc.identifier.otherPURE UUID: c893ad28-c22a-4d22-951c-b24045b1c300
dc.identifier.otherScopus: 85076798846
dc.identifier.otherWOS: 000506637600043
dc.identifier.otherPubMed: 31671536
dc.identifier.otherORCID: /0000-0003-0313-0778/work/124583114
dc.identifier.urihttp://hdl.handle.net/10362/116623
dc.identifier.urlhttps://www.scopus.com/pages/publications/85076798846
dc.language.isoeng
dc.peerreviewedyes
dc.subjectELISA
dc.subjectMalaria
dc.subjectPlasmodium falciparum
dc.subjectSerological diagnosis
dc.subjectSerological markers
dc.subjectClinical Biochemistry
dc.subjectImmunology
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleComparative analysis of the serological reactivity of individuals with clinical history of malaria using two different ELISA testsen
dc.typejournal article
degois.publication.issue4
degois.publication.titleDiagnostics
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

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