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Genetic competence drives genome diversity in Bacillus subtilis

2018-01Artigo científico Acesso aberto
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dc.contributor.authorBrito, Patrícia H
dc.contributor.authorChevreux, Bastien
dc.contributor.authorSerra, Cláudia R
dc.contributor.authorSchyns, Ghislain
dc.contributor.authorHenriques, Adriano O
dc.contributor.authorPereira-Leal, José B
dc.contributor.authorPereira-Leal, José
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblOxford University Press
dc.date.accessioned2018-03-20T23:13:46Z
dc.date.available2018-03-20T23:13:46Z
dc.date.issued2018-01
dc.description© The Author(s) 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.
dc.description.abstractProkaryote genomes are the result of a dynamic flux of genes, with increases achieved via horizontal gene transfer and reductions occurring through gene loss. The ecological and selective forces that drive this genomic flexibility vary across species. Bacillus subtilis is a naturally competent bacterium that occupies various environments, including plant-associated, soil, and marine niches, and the gut of both invertebrates and vertebrates. Here, we quantify the genomic diversity of B. subtilis and infer the genome dynamics that explain the high genetic and phenotypic diversity observed. Phylogenomic and comparative genomic analyses of 42 B. subtilis genomes uncover a remarkable genome diversity that translates into a core genome of 1659 genes and an asymptotic pangenome growth rate of 57 new genes per new genome added. This diversity is due to a large proportion of low-frequency genes that are acquired from closely related species. We find no gene-loss bias among wild isolates, which explains why the cloud genome, 43% of the species pangenome, represents only a small proportion of each genome. We show that B. subtilis can acquire xenologous copies of core genes that propagate laterally among strains within a niche. While not excluding the contributions of other mechanisms, our results strongly suggest a process of gene acquisition that is largely driven by competence, where the long-term maintenance of acquired genes depends on local and global fitness effects. This competence-driven genomic diversity provides B. subtilis with its generalist character, enabling it to occupy a wide range of ecological niches and cycle through them.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent17
dc.format.extent1597738
dc.identifier.doi10.1093/gbe/evx270
dc.identifier.otherPURE: 3409545
dc.identifier.otherPURE UUID: 725c99d5-2c62-4455-979c-a59c6cf8198a
dc.identifier.otherPubMed: 29272410
dc.identifier.otherWOS: 000424893500009
dc.identifier.otherScopus: 85045936343
dc.identifier.otherORCID: /0000-0002-2457-7520/work/151431805
dc.identifier.urihttp://hdl.handle.net/10362/32936
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBacillus subtilis
dc.subjectpangenome
dc.subjectcomparative genomics
dc.subjectbacterial genome evolution
dc.subjectlateral gene transfer
dc.subjectgenetic competence
dc.subjectSDG 14 - Life Below Water
dc.titleGenetic competence drives genome diversity in Bacillus subtilisen
dc.typejournal article
degois.publication.firstPage108
degois.publication.issue1
degois.publication.lastPage124
degois.publication.titleGenome Biology and Evolution
degois.publication.volume10
dspace.entity.typePublication
person.familyNamePereira Leal
person.givenNameJose
person.identifier.ciencia-idA819-D3B9-A416
person.identifier.orcid0000-0002-3111-7119
person.identifier.scopus-author-id55940842900
rcaap.rightsopenAccess
relation.isAuthorOfPublication39ace361-72b9-4bd9-aa4d-358de4a24b76
relation.isAuthorOfPublication.latestForDiscovery39ace361-72b9-4bd9-aa4d-358de4a24b76

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