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Low temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymer

2020-03-14Artigo científico Acesso aberto
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dc.contributor.authorAraújo, Diana
dc.contributor.authorAlves, Vítor D.
dc.contributor.authorMarques, Ana C.
dc.contributor.authorFortunato, Elvira
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorFreitas, Filomena
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
dc.contributor.institutionCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.pblMDPI AG
dc.date.accessioned2021-03-23T23:24:25Z
dc.date.available2021-03-23T23:24:25Z
dc.date.issued2020-03-14
dc.descriptionUIDB/04378/2020 UID/AGR/04129/2020 UID/CTM/50025/2019 BD/140829/2018 SFRH/BD/115173/2016
dc.description.abstractChitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first time for solubilization of CGC extracted from the yeast Komagataella pastoris. Different NaOH/urea ratios (6:8, 8:4 and 11:4 (w/w), respectively) were used to obtain aqueous solutions using a freeze/thaw procedure. There was an overall solubilization of 63–68%, with the highest solubilization rate obtained for the highest tested urea concentration (8 wt%). The regenerated polymer, obtained by dialysis of the alkali solutions followed by lyophilization, formed porous macrostructures characterized by a chemical composition similar to that of the starting co-polymer, although the acetylation degree decreased from 61.3% to 33.9–50.6%, indicating that chitin was converted into chitosan, yielding chitosan-glucan complex (ChGC). Consistent with this, there was a reduction of the crystallinity index and thermal degradation temperature. Given these results, this study reports a simple and green procedure to solubilize CGC and obtain aqueous ChGC solutions that can be processed as novel biomaterials.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1983977
dc.identifier.doi10.3390/bioengineering7010028
dc.identifier.otherPURE: 18017744
dc.identifier.otherPURE UUID: d8dd76c5-17cd-4c38-b0ab-e0ca1b4dd9e9
dc.identifier.otherScopus: 85082088296
dc.identifier.otherPubMed: 32183337
dc.identifier.otherPubMedCentral: PMC7175172
dc.identifier.otherORCID: /0000-0002-9430-4640/work/91113059
dc.identifier.otherORCID: /0000-0002-4202-7047/work/91113321
dc.identifier.urihttp://hdl.handle.net/10362/114326
dc.identifier.urlhttps://www.scopus.com/pages/publications/85082088296
dc.language.isoeng
dc.peerreviewedyes
dc.subjectChitin-glucan complex (CGC)
dc.subjectChitosan-glucan complex (ChGC)
dc.subjectDissolution
dc.subjectNaOH/urea solvent systems
dc.subjectStructural analysis
dc.subjectThermal properties
dc.subjectBioengineering
dc.titleLow temperature dissolution of yeast Chitin-Glucan complex and characterization of the regenerated polymeren
dc.typejournal article
degois.publication.issue1
degois.publication.titleBioengineering
degois.publication.volume7
dspace.entity.typePublication
rcaap.rightsopenAccess

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