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Proteomic analysis of apoplastic fluid of Coffea arabica leaves highlights novel biomarkers for resistance against Hemileia vastatrix

2015-06-30Artigo científico Acesso aberto
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dc.contributor.authorGuerra-Guimarães, Leonor
dc.contributor.authorTenente, Rita
dc.contributor.authorPinheiro, Carla
dc.contributor.authorChaves, Inês
dc.contributor.authorSilva, Maria do Céu
dc.contributor.authorCardoso, Fernando M H
dc.contributor.authorPlanchon, Sébastien
dc.contributor.authorBarros, Danielle R.
dc.contributor.authorRenaut, Jenny
dc.contributor.authorRicardo, Cândido P.
dc.contributor.authorRicardo, C. P.
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.pblFrontiers Media
dc.date.accessioned2018-05-14T22:01:49Z
dc.date.available2018-05-14T22:01:49Z
dc.date.issued2015-06-30
dc.descriptionPMID: 26175744 WOS:000358419400001
dc.description.abstractA proteomic analysis of the apoplastic fluid (APF) of coffee leaves was conducted to investigate the cellular processes associated with incompatible (resistant) and compatible (susceptible) Coffea arabica-Hemileia vastatrix interactions, during the 24–96 hai period. The APF proteins were extracted by leaf vacuum infiltration and protein profiles were obtained by 2-DE. The comparative analysis of the gels revealed 210 polypeptide spots whose volume changed in abundance between samples (control, resistant and susceptible) during the 24–96 hai period. The proteins identified were involved mainly in protein degradation, cell wall metabolism and stress/defense responses, most of them being hydrolases (around 70%), particularly sugar hydrolases and peptidases/proteases. The changes in the APF proteome along the infection process revealed two distinct phases of defense responses, an initial/basal one (24–48 hai) and a late/specific one (72–96 hai). Compared to susceptibility, resistance was associated with a higher number of proteins, which was more evident in the late/specific phase. Proteins involved in the resistance response were mainly, glycohydrolases of the cell wall, serine proteases and pathogen related-like proteins (PR-proteins), suggesting that some of these proteins could be putative candidates for resistant markers of coffee to H. vastatrix. Antibodies were produced against chitinase, pectin methylesterase, serine carboxypeptidase, reticuline oxidase and subtilase and by an immunodetection assay it was observed an increase of these proteins in the resistant sample. With this methodology we have identified proteins that are candidate markers of resistance and that will be useful in coffee breeding programs to assist in the selection of cultivars with resistance to H. vastatrix.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1135153
dc.identifier.doi10.3389/fpls.2015.00478
dc.identifier.issn1664-462X
dc.identifier.otherPURE: 1716397
dc.identifier.otherPURE UUID: 2b1a0963-0a31-49a5-814f-402b27908e3b
dc.identifier.otherScopus: 84936952879
dc.identifier.otherWOS: 000358419400001
dc.identifier.otherPubMed: 26175744
dc.identifier.otherORCID: /0000-0002-7799-0183/work/53556881
dc.identifier.otherORCID: /0000-0003-1501-9479/work/69039031
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=84936952879&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/84936952879
dc.language.isoeng
dc.peerreviewedyes
dc.subject2-DE
dc.subjectAntibody production
dc.subjectCoffee leaf rust (CLR)
dc.subjectCytology
dc.subjectELISA assay
dc.subjectMALDI-TOF/TOF MS
dc.subjectPlant Science
dc.titleProteomic analysis of apoplastic fluid of Coffea arabica leaves highlights novel biomarkers for resistance against Hemileia vastatrixen
dc.typejournal article
degois.publication.issueJUNE
degois.publication.titleFrontiers in Plant Science
degois.publication.volume6
dspace.entity.typePublication
rcaap.rightsopenAccess

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