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Enzyme immunoassays (EIA) for serodiagnosis of human leptospirosis

2022-02Artigo científico Acesso aberto
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dc.contributor.authorFortes-Gabriel, Elsa
dc.contributor.authorGuedes, Mariana Soares
dc.contributor.authorShetty, Advait
dc.contributor.authorGomes, Charles Klazer
dc.contributor.authorCarreira, Teresa
dc.contributor.authorVieira, Maria Luísa
dc.contributor.authorEsteves, Lisa
dc.contributor.authorMota-Vieira, Luísa
dc.contributor.authorGomes-Solecki, Maria
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2022-03-14T23:23:31Z
dc.date.available2022-03-14T23:23:31Z
dc.date.issued2022-02
dc.description.abstractThe laborious microscopic agglutination test (MAT) is the gold standard serologic test for laboratory diagnosis of leptospirosis. We developed EIA based serologic assays using recombinant proteins (rLigA, rLigB, rLipL32) and whole-cell extracts from eight Leptospira serovars as antigen and assessed the diagnostic performance of the new assay within each class, against MAT positive (MAT+) human sera panels from Portugal/PT (n = 143) and Angola/AO (n = 100). We found that a combination of recombinant proteins rLigA, rLigB and rLipL32 correctly identified antigen-specific IgG from patients with clinical and laboratory confirmed leptospirosis (MAT+) with 92% sensitivity and ~ 97% specificity (AUC 0.974) in serum from the provinces of Luanda (LDA) and Huambo (HBO) in Angola. A combination of whole cell extracts of L. interrogans sv Copenhageni (LiC), L. kirschneri Mozdok (LkM), L. borgpetersenii Arborea (LbA) and L. biflexa Patoc (LbP) accurately identified patients with clinical and laboratory confirmed leptospirosis (MAT+) with 100% sensitivity and ~ 98% specificity for all provinces of Angola and Portugal (AUC: 0.997 for AO/LDA/HBO, 1.000 for AO/HLA, 0.999 for PT/AZ and 1.000 for PT/LIS). Interestingly, we found that MAT+ IgG+ serum from Angola had a significantly higher presence of IgD and that IgG3/IgG1 isotypes were significantly increased in the MAT+ IgG+ serum from Portugal. Given that IgM/IgD class and IgG3/IgG1 specific isotypes are produced in the earliest course of infection, immunoglobulin G isotyping may be used to inform diagnosis of acute leptospirosis. The speed, ease of use and accuracy of EIA tests make them excellent alternatives to the laborious and expensive MAT for screening acute infection in areas where circulating serovars of pathogenic Leptospira are well defined.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1565732
dc.identifier.doi10.1371/journal.pntd.0010241
dc.identifier.issn1935-2727
dc.identifier.otherPURE: 42289212
dc.identifier.otherPURE UUID: 32f98c60-ef2f-4bda-bbf1-d855ab8fb2d0
dc.identifier.otherPubMed: 35196321
dc.identifier.otherScopus: 85125966284
dc.identifier.otherWOS: 000761004700002
dc.identifier.otherORCID: /0000-0002-8586-5534/work/151385542
dc.identifier.urihttp://hdl.handle.net/10362/134499
dc.identifier.urlhttps://www.scopus.com/pages/publications/85125966284
dc.language.isoeng
dc.peerreviewedyes
dc.subjectPublic Health, Environmental and Occupational Health
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleEnzyme immunoassays (EIA) for serodiagnosis of human leptospirosisen
dc.title.subtitleSpecific IgG3/IgG1 isotyping may further inform diagnosis of acute diseaseen
dc.typejournal article
degois.publication.firstPage
degois.publication.issue2
degois.publication.lastPage
degois.publication.titlePLoS Neglected Tropical Diseases
degois.publication.volume16
dspace.entity.typePublication
rcaap.rightsopenAccess

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