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Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics

2020-05-01Artigo científico Acesso aberto
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dc.contributor.authorSantos, Ana Isabel
dc.contributor.authorLourenço, Ana Sofia
dc.contributor.authorSimão, Sónia
dc.contributor.authorMarques da Silva, Dorinda
dc.contributor.authorSantos, Daniela Filipa
dc.contributor.authorOnofre de Carvalho, Ana Paula
dc.contributor.authorPereira, Ana Catarina
dc.contributor.authorIzquierdo-Álvarez, Alicia
dc.contributor.authorRamos, Elena
dc.contributor.authorMorato, Esperanza
dc.contributor.authorMarina, Anabel
dc.contributor.authorMartínez-Ruiz, Antonio
dc.contributor.authorAraújo, Inês Maria
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblElsevier
dc.date.accessioned2020-12-30T00:29:27Z
dc.date.available2020-12-30T00:29:27Z
dc.date.issued2020-05-01
dc.descriptionFunding: The authors acknowledge the kind gift of HC7 cells by Dr. Fred H. Gage (The Salk Institute for Biological Studies). The excellent assistance of Pedro Marques Vilela in graphical artwork is acknowledged by the authors. Ana Santos and Ana Lourenço were supported by Foundation for Science and Technology, I.P. (FCT, Portugal; fellowships SFRH/BD/ 77903/2011 and SFRH/BD/79308/2011) and are PhD students of the PhD programme in Biomedical Sciences of the University of Algarve. Antonio Martínez-Ruiz is supported by the ISCIII from the Spanish Government (IS3SNS programme, partially funded by FEDER/ERDF). Inês Araújo is supported by FEDER/ERDF funds via Programa Operacional Factores de Competitividade (COMPETE), by national funds via FCT (grants PTDC/NEU-OSD/0473/2012, PTDC/QUI-QFI/ 29319/2017) and by regional funds via CRESC ALGARVE 2020 (grant UID/BIM/04773/2019), by the Algarve Biomedical Center (ABC) and Loulé Municipality. This work was supported by the COST action BM1005 (ENOG: European Network on Gasotransmitters), by the Spanish Government (grants PS09/00101, PI12/00875 and PI15/ 00107 from ISCIII and RTI2018-094203-B-I00 from AEI; co-financed by FEDER/ERDF) and by the Spanish-Portuguese Integrated Action grant PRI-AIBPT-2011-1015/E-10/12. The Proteomics Service of the CBMSO is a member of ProteoRed (PRB3-ISCIII), and is supported by grant PT13/0001/0024 of Spanish Government (cofinanced by FEDER/ ERDF).
dc.description.abstractNitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R–SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2093948
dc.identifier.doi10.1016/j.redox.2020.101457
dc.identifier.issn2213-2317
dc.identifier.otherPURE: 17839972
dc.identifier.otherPURE UUID: dc9638bb-4b68-47d1-be51-6f29d4eda300
dc.identifier.otherScopus: 85079639048
dc.identifier.otherPubMed: 32088623
dc.identifier.otherWOS: 000537459900022
dc.identifier.urihttp://hdl.handle.net/10362/109469
dc.identifier.urlhttps://www.scopus.com/pages/publications/85079639048
dc.language.isoeng
dc.peerreviewedyes
dc.subjectHippocampus
dc.subjectNeural stem cells
dc.subjectNeurogenesis
dc.subjectNitric oxide
dc.subjectS-nitrosylation
dc.subjectSeizures
dc.subjectOrganic Chemistry
dc.subjectClinical Biochemistry
dc.titleIdentification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomicsen
dc.typejournal article
degois.publication.titleRedox Biology
degois.publication.volume32
dspace.entity.typePublication
rcaap.rightsopenAccess

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