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Polyhydroxyalkanoates from A Mixed Microbial Culture

2022-05-25Artigo científico Acesso aberto
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dc.contributor.authorRodrigues, Ana Marta
dc.contributor.authorFranca, Rita Dias Guardão
dc.contributor.authorDionísio, Madalena
dc.contributor.authorSevrin, Chantal
dc.contributor.authorGrandfils, Christian
dc.contributor.authorReis, Maria A. M.
dc.contributor.authorLourenço, Nídia Dana
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-09-21T22:37:53Z
dc.date.available2022-09-21T22:37:53Z
dc.date.issued2022-05-25
dc.descriptionFunding: This work was funded by the INGREEN project that has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 838120. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio-based Industries Consortium. This work was financed by national funds from FCT—Fundação para a Ciência e a Tecno-logia, I.P., in the scope of the project and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. A.M. Rodrigues acknowledges the financial support of FCT through the PhD grant 2020.08574.BD. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractPolyhydroxyalkanoates (PHA) are biopolymers with potential to replace conventional oil-based plastics. However, PHA high production costs limit their scope of commercial applications. Downstream processing is currently the major cost factor for PHA production but one of the least investigated aspects of the PHA production chain. In this study, the extraction of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) produced at pilot scale by a mixed microbial culture was performed using sodium hydroxide (NaOH) or sodium hypochlorite (NaClO) as digestion agents of non-PHA cellular mass. Optimal conditions for digestion with NaOH (0.3 M, 4.8 h) and NaClO (9.0%, 3.4 h) resulted in polymers with a PHA purity and recovery of ca. 100%, in the case of the former and ca. 99% and 90%, respectively, in the case of the latter. These methods presented higher PHA recoveries than extraction by soxhlet with chloroform, the benchmark protocol for PHA extraction. The polymers extracted by the three methods presented similar PHA purities, molecular weights and polydispersity indices. Using the optimized conditions for NaOH and NaClO digestions, this study analyzed the effect of the initial intracellular PHA content (40–70%), biomass concentration (20–100 g/L) and biomass pre-treatment (fresh vs. dried vs. lyophilized) on the performance of PHA extraction by these two methods.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent22
dc.format.extent4277494
dc.identifier.doi10.3390/polym14112155
dc.identifier.otherPURE: 46647992
dc.identifier.otherPURE UUID: a52218b2-aea7-4d53-b028-9825f15a9a75
dc.identifier.otherScopus: 85131367965
dc.identifier.otherWOS: 000809147700001
dc.identifier.otherPubMed: 35683828
dc.identifier.otherPubMedCentral: PMC9182939
dc.identifier.otherORCID: /0000-0002-1487-0889/work/119457894
dc.identifier.urihttp://hdl.handle.net/10362/143926
dc.identifier.urlhttps://www.scopus.com/pages/publications/85131367965
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
dc.relationThis work was funded by the INGREEN project that has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No 838120. The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio-based Industries Consortium. This work was financed by national funds from Research Unit on Applied Molecular Biosciences—UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. A.M. Rodrigues acknowledges the financial support of FCT through the PhD grant 2020.08574.BD.Acknowledgments: The authors acknowledge Cristiana Torres for her support in the CCRD. Funding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.subjectchemical digestion
dc.subjectdesign of experiments
dc.subjectPHA extraction
dc.subjectpolymer properties
dc.subjectGeneral Chemistry
dc.subjectPolymers and Plastics
dc.titlePolyhydroxyalkanoates from A Mixed Microbial Cultureen
dc.title.subtitleExtraction Optimization and Polymer Characterizationen
dc.typejournal article
degois.publication.issue11
degois.publication.titlePolymers
degois.publication.volume14
dspace.entity.typePublication
oaire.awardNumberUIDB/04378/2020
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicatione07cf232-4705-4b5b-b2c4-af8f25311076
relation.isProjectOfPublication.latestForDiscoverye07cf232-4705-4b5b-b2c4-af8f25311076

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