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Hyperthermia induced by gold nanoparticles and visible light photothermy combined with chemotherapy to tackle doxorubicin sensitive and resistant colorectal tumor 3D spheroids

2020-10-28Artigo científico Acesso aberto
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dc.contributor.authorRoma-Rodrigues, Catarina
dc.contributor.authorPombo, Inês
dc.contributor.authorFernandes, Alexandra R.
dc.contributor.authorBaptista, Pedro V.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-04-08T22:16:49Z
dc.date.available2021-04-08T22:16:49Z
dc.date.issued2020-10-28
dc.descriptionUIDB/04378/2020 SFRH/BPD/124612/2016
dc.description.abstractCurrent cancer therapies are frequently ineffective and associated with severe side effects and with acquired cancer drug resistance. The development of effective therapies has been hampered by poor correlations between pre-clinical and clinical outcomes. Cancer cell-derived spheroids are three-dimensional (3D) structures that mimic layers of tumors in terms of oxygen and nutrient and drug resistance gradients. Gold nanoparticles (AuNP) are promising therapeutic agents which permit diminishing the emergence of secondary effects and increase therapeutic efficacy. In this work, 3D spheroids of Doxorubicin (Dox)-sensitive and -resistant colorectal carcinoma cell lines (HCT116 and HCT116-DoxR, respectively) were used to infer the potential of the combination of chemotherapy and Au-nanoparticle photothermy in the visible (green laser of 532 nm) to tackle drug resistance in cancer cells. Cell viability analysis of 3D tumor spheroids suggested that AuNPs induce cell death in the deeper layers of spheroids, further potentiated by laser irradiation. The penetration of Dox and earlier spheroid disaggregation is potentiated in combinatorial therapy with Dox, AuNP functionalized with polyethylene glycol (AuNP@PEG) and irradiation. The time point of Dox administration and irradiation showed to be important for spheroids destabilization. In HCT116-sensitive spheroids, pre-irradiation induced earlier disintegration of the 3D structure, while in HCT116 Dox-resistant spheroids, the loss of spheroid stability occurred almost instantly in post-irradiated spheroids, even with lower Dox concentrations. These results point towards the application of new strategies for cancer therapeutics, reducing side effects and resistance acquisition.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent13
dc.format.extent2703275
dc.identifier.doi10.3390/ijms21218017
dc.identifier.issn1661-6596
dc.identifier.otherPURE: 26306095
dc.identifier.otherPURE UUID: 55c75cd0-73e4-4065-b1d2-034fd994d48e
dc.identifier.otherScopus: 85094934516
dc.identifier.otherPubMed: 33126535
dc.identifier.otherPubMedCentral: PMC7672550
dc.identifier.otherWOS: 000589127400001
dc.identifier.otherORCID: /0000-0001-5255-7095/work/91873579
dc.identifier.otherORCID: /0000-0003-2054-4438/work/91874024
dc.identifier.urihttp://hdl.handle.net/10362/115246
dc.identifier.urlhttps://www.scopus.com/pages/publications/85094934516
dc.language.isoeng
dc.peerreviewedyes
dc.subject3D spheroids
dc.subjectColorectal cancer
dc.subjectDoxorubicin resistance
dc.subjectGold nanoparticles
dc.subjectPhotothermy
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleHyperthermia induced by gold nanoparticles and visible light photothermy combined with chemotherapy to tackle doxorubicin sensitive and resistant colorectal tumor 3D spheroidsen
dc.typejournal article
degois.publication.firstPage1
degois.publication.issue21
degois.publication.lastPage13
degois.publication.titleInternational Journal of Molecular Sciences
degois.publication.volume21
dspace.entity.typePublication
rcaap.rightsopenAccess

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