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Natural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiation

2020-08-10Artigo científico Acesso aberto
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dc.contributor.authorFernandes, Cláudia S. M.
dc.contributor.authorRodrigues, André L.
dc.contributor.authorAlves, Vitor D.
dc.contributor.authorFernandes, Tiago G.
dc.contributor.authorPina, Ana Sofia
dc.contributor.authorRoque, Ana Cecília A.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.pblACS - American Chemical Society
dc.date.accessioned2022-07-13T22:20:35Z
dc.date.available2022-07-13T22:20:35Z
dc.date.issued2020-08-10
dc.descriptionFCT/MEC (PTDC/BII-BIO/28878/2017; UIDB/04378/2020; UID/QUI/50006/2019; UID/AGR/04129/2019; POCI-01-0145FEDER-007728; LISBOA-01-0145-FEDER-028878); PD/BD/105871/2014; PD/BD/135524/2018;
dc.description.abstractTissue engineering and stem cell research greatly benefit from cell encapsulation within hydrogels as it promotes cell expansion and differentiation. Affinity-triggered hydrogels, an appealing solution for mild cell encapsulation, rely on selective interactions between the ligand and target and also on the multivalent presentation of these two components. Although these hydrogels represent a versatile option to generate dynamic, tunable, and highly functional materials, the design of hydrogel properties based on affinity and multivalency remains challenging and unstudied. Here, the avidin-biotin affinity pair, with the highest reported affinity constant, is used to address this challenge. It is demonstrated that the binding between the affinity hydrogel components is influenced by the multivalent display selected. In addition, the natural multivalency of the interaction must be obeyed to yield robust multicomponent synthetic protein hydrogels. The hydrogel's resistance to erosion depends on the right stoichiometric match between the hydrogel components. The developed affinity-triggered hydrogels are biocompatible and support encapsulation of induced pluripotent stem cells and their successful differentiation into a neural cell line. This principle can be generalized to other affinity pairs using multimeric proteins, yielding biomaterials with controlled performance.en
dc.description.versionauthorsversion
dc.description.versionpublished
dc.format.extent11
dc.format.extent6943048
dc.identifier.doi10.1021/acs.biomac.0c00473
dc.identifier.issn1525-7797
dc.identifier.otherPURE: 19976147
dc.identifier.otherPURE UUID: fbed8692-25e8-45ad-a872-dfd6fdee891d
dc.identifier.otherScopus: 85089615165
dc.identifier.otherPubMed: 32573205
dc.identifier.otherWOS: 000562121800011
dc.identifier.otherORCID: /0000-0001-9729-0371/work/90657553
dc.identifier.urihttp://hdl.handle.net/10362/141852
dc.identifier.urlhttps://www.scopus.com/pages/publications/85089615165
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F97585%2F2013/PT
dc.subjectBioengineering
dc.subjectBiomaterials
dc.subjectPolymers and Plastics
dc.subjectMaterials Chemistry
dc.titleNatural Multimerization Rules the Performance of Affinity-Based Physical Hydrogels for Stem Cell Encapsulation and Differentiationen
dc.typejournal article
degois.publication.firstPage3081
degois.publication.issue8
degois.publication.lastPage3091
degois.publication.titleBiomacromolecules
degois.publication.volume21
dspace.entity.typePublication
oaire.awardNumberSFRH/BPD/97585/2013
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F97585%2F2013/PT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicationb3f32819-831f-4ee8-95b7-a57370857eb5
relation.isProjectOfPublication.latestForDiscoveryb3f32819-831f-4ee8-95b7-a57370857eb5

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