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Aneuploidy and high S-phase as biomarkers of poor clinical outcome in poorly differentiated and anaplastic thyroid carcinoma

2008-10Artigo científico Acesso aberto
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dc.contributor.authorPinto, António E.
dc.contributor.authorSilva, Giovani
dc.contributor.authorBanito, Ana
dc.contributor.authorLeite, Valeriano
dc.contributor.authorSoares, Jorge
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblSpandidos Publications
dc.date.accessioned2022-07-19T22:20:26Z
dc.date.available2022-07-21T00:31:29Z
dc.date.embargoedUntil2022-07-20
dc.date.issued2008-10
dc.description.abstractThis study aimed to investigate the prognostic influence of DNA flow cytometry and RAS gene mutations in patients with poorly differentiated (PDTC) and anaplastic thyroid carcinoma (ATC). The series consisted of 26 patients with PDTC and ATC, and a median follow-up of 10 months (range 1-138). DNA ploidy and S-phase fraction (SPF) were assessed by flow cytometry on frozen samples. RAS point mutations were detected using PCR techniques. Disease staging and tumour angioinvasion were included as prognostic parameters for survival analysis. Nineteen patients (73.1%) succumbed to the disease (median time 5 months; range 1-45). Eighteen tumours (69.2%) were classified as DNA aneuploid. Median SPF was 5.6% (range 1.9-23.1), which was used as a cut-off value to distinguish between low versus high cell proliferation. Three of 20 (15%) patients presented N-RAS gene mutations in codon 61. DNA aneuploidy was most frequently found in female patients (p=0.034). Kaplan-Meier and Cox regression analyses showed that only DNA aneuploidy (p=0.044 and p=0.055, respectively) and high SPF (p=0.001 and p=0.006, respectively) significantly correlated with worse survival. The results indicate that aneuploidy and high SPF are biomarkers of poor clinical outcome in PDTC and ATC, which may provide useful prognostic information with a potentially therapeutic impact in patient management.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent7
dc.format.extent214982
dc.identifier.doi10.3892/or_00000091
dc.identifier.issn1021-335X
dc.identifier.otherPURE: 3152340
dc.identifier.otherPURE UUID: 19c72bf5-55fa-4913-ac33-cdbaca49e720
dc.identifier.otherScopus: 54949150226
dc.identifier.otherPubMed: 18813835
dc.identifier.otherWOS: 000259767400030
dc.identifier.urihttp://hdl.handle.net/10362/142149
dc.identifier.urlhttps://www.scopus.com/pages/publications/54949150226
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAnaplastic thyroid carcinoma
dc.subjectDNA ploidy
dc.subjectPoorly differentiated thyroid carcinoma
dc.subjectPrognosis
dc.subjectRAS mutations
dc.subjectS-phase fraction
dc.subjectCancer Research
dc.subjectOncology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleAneuploidy and high S-phase as biomarkers of poor clinical outcome in poorly differentiated and anaplastic thyroid carcinomaen
dc.typejournal article
degois.publication.firstPage913
degois.publication.issue4
degois.publication.lastPage919
degois.publication.titleOncology Reports
degois.publication.volume20
dspace.entity.typePublication
rcaap.rightsopenAccess

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