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Multigene assessment of the species boundaries and sexual status of the basidiomycetous yeasts Cryptococcus flavescens and C. terrestris (Tremellales)

2015-03-26Artigo científico Acesso aberto
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dc.contributor.authorYurkov, Andrey M.
dc.contributor.authorGuerreiro, Marco A.
dc.contributor.authorSharma, Lav
dc.contributor.authorCarvalho, Cláudia
dc.contributor.authorFonseca, Álvaro
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.pblPLOS - Public Library of Science
dc.date.accessioned2017-09-18T22:02:53Z
dc.date.available2017-09-18T22:02:53Z
dc.date.issued2015-03-26
dc.descriptionThis work was supported by funds from FCT-Fundacao para a Ciencia e a Tecnologia, Portugal (www.fct.pt), project PTDC/BIA-MIC/113051/2009. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
dc.description.abstractCryptococcus flavescens and C. terrestris are phenotypically indistinguishable sister species that belong to the order Tremellales (Tremellomycetes, Basidiomycota) and which may be mistaken for C. laurentii based on phenotype. Phylogenetic separation between C. flavescens and C. terrestris was based on rDNA sequence analyses, but very little is known on their intraspecific genetic variability or propensity for sexual reproduction. We studied 59 strains from different substrates and geographic locations, and used a multilocus sequencing (MLS) approach complemented with the sequencing of mating type (MAT) genes to assess genetic variation and reexamine the boundaries of the two species, as well as their sexual status. The following five loci were chosen for MLS: the rDNA ITS-LSU region, the rDNA IGS1 spacer, and fragments of the genes encoding the largest subunit of RNA polymerase II (RPB1), the translation elongation factor 1 alpha (TEF1) and the p21-activated protein kinase (STE20). Phylogenetic network analyses confirmed the genetic separation of the two species and revealed two additional cryptic species, for which the names Cryptococcus baii and C. ruineniae are proposed. Further analyses of the data revealed a high degree of genetic heterogeneity within C. flavescens as well as evidence for recombination between lineages detected for this species. Strains of C. terrestris displayed higher levels of similarity in all analysed genes and appear to make up a single recombining group. The two MAT genes (STE3 and SXI1/SXI2) sequenced for C. flavescens strains confirmed the potential for sexual reproduction and suggest the presence of a tetrapolar mating system with a biallelic pheromone/receptor locus and a multiallelic HD locus. In C. terrestris we could only sequence STE3, which revealed a biallelic P/R locus. In spite of the strong evidence for sexual recombination in the two species, attempts at mating compatible strains of both species on culture media were unsuccessful.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1215882
dc.identifier.doi10.1371/journal.pone.0120400
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 2898545
dc.identifier.otherPURE UUID: d5b8c1c9-6678-4e14-9e51-a67a79f7deb2
dc.identifier.otherScopus: 84926171291
dc.identifier.otherPubMed: 25811603
dc.identifier.otherWOS: 000356353700048
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=84926171291&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/84926171291
dc.language.isoeng
dc.peerreviewedyes
dc.subjectINTERNAL TRANSCRIBED SPACER
dc.subjectMULTIPLE SEQUENCE ALIGNMENT
dc.subjectPHYLOGENETIC ANALYSIS
dc.subjectGENETIC-EVIDENCE
dc.subjectNOV TREMELLALES
dc.subjectNUCLEAR LARGE
dc.subjectMATING-TYPE
dc.subjectNEOFORMANS
dc.subjectRECOMBINATION
dc.subjectEVOLUTION
dc.subjectGeneral Medicine
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectGeneral Agricultural and Biological Sciences
dc.titleMultigene assessment of the species boundaries and sexual status of the basidiomycetous yeasts Cryptococcus flavescens and C. terrestris (Tremellales)en
dc.typejournal article
degois.publication.issue3
degois.publication.titlePLoS ONE
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

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