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Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens

2024-10-19Artigo científico Acesso aberto
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dc.contributor.authorHerrando, Alberto Ignacio
dc.contributor.authorFernandez, Laura M.
dc.contributor.authorAzevedo, José
dc.contributor.authorVieira, Pedro
dc.contributor.authorDomingos, Hugo
dc.contributor.authorGalzerano, Antonio
dc.contributor.authorShcheslavskiy, Vladislav
dc.contributor.authorHeald, Richard J.
dc.contributor.authorParvaiz, Amjad
dc.contributor.authorda Silva, Pedro Garcia
dc.contributor.authorCastillo-Martin, Mireia
dc.contributor.authorLagarto, João L.
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblNature Publishing Group
dc.date.accessioned2024-12-18T17:19:36Z
dc.date.available2024-12-18T17:19:36Z
dc.date.issued2024-10-19
dc.description.abstractColorectal cancer (CRC) ranks among the most prevalent malignancies worldwide, driving a quest for comprehensive characterization methods. We report a characterization of the ex vivo autofluorescence lifetime fingerprint of colorectal tissues obtained from 73 patients that underwent surgical resection. We specifically target the autofluorescence characteristics of collagens, reduced nicotine adenine (phosphate) dinucleotide (NAD(P)H), and flavins employing a fiber-based dual excitation (375 nm and 445 nm) optical imaging system. Autofluorescence-derived parameters obtained from normal tissues, adenomatous lesions, and adenocarcinomas were analyzed considering the underlying clinicopathological features. Our results indicate that differences between tissues are primarily driven by collagen and flavins autofluorescence parameters. We also report changes in the autofluorescence parameters associated with NAD(P)H that we tentatively attribute to intratumoral heterogeneity, potentially associated to the presence of distinct metabolic subpopulations. Changes in autofluorescence signatures of malignant tumors were also observed with lymphatic and venous invasion, differentiation grade, and microsatellite instability. Finally, we characterized the impact of radiative treatment in the autofluorescence fingerprints of rectal tissues and observed a generalized increase in the mean lifetime of radiated adenocarcinomas, which is suggestive of altered metabolism and structural remodeling. Overall, our preliminary findings indicate that multiparametric autofluorescence lifetime measurements have the potential to significantly enhance clinical decision-making in CRC, spanning from initial diagnosis to ongoing management. We believe that our results will provide a foundational framework for future investigations to further understand and combat CRC exploiting autofluorescence measurements.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1
dc.format.extent5430887
dc.identifier.doi10.1038/s41598-024-74224-8
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 102174513
dc.identifier.otherPURE UUID: 249a7390-476e-4b8e-b88a-55324a43e86d
dc.identifier.otherScopus: 85206872077
dc.identifier.otherPubMed: 39426971
dc.identifier.urihttp://hdl.handle.net/10362/176455
dc.identifier.urlhttps://www.scopus.com/pages/publications/85206872077
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral
dc.subjectSDG 3 - Good Health and Well-being
dc.titleDetection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimensen
dc.typejournal article
degois.publication.issue1
degois.publication.titleScientific Reports
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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