Logo do repositório
 
Publicação

Zero-waste extraction of polyhydroxy(butyrate-co-valerate) (PHBV) from mixed cultures using natural deep eutectic solvents (NADES)

2025-05-01Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorDidion, Yannick Patrice
dc.contributor.authorTjalsma, Tjalling
dc.contributor.authorMalankowska, Magdalena
dc.contributor.authorSu, Ziran
dc.contributor.authorMatos, Mariana
dc.contributor.authorPinelo, Manuel
dc.contributor.authorCrespo, Joao
dc.contributor.authorBrazinha, Carla
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.pblElsevier Science Publisher B.V.
dc.date.accessioned2025-07-21T21:13:47Z
dc.date.available2025-07-21T21:13:47Z
dc.date.issued2025-05-01
dc.descriptionFunding Information: This work was funded by the Novo Nordisk Foundation within the framework of the Fermentation-based Biomanufacturing Initiative (FBM), grant number: NNF17SA0031362. Furthermore, the authors gratefully acknowledge the PhD students Jorge Viana, Suchintan Mondal and technician Nuno Costa (Universidade NOVA de Lisboa, Faculdade de Ciências e Tecnologia (FCT)), for their help in sample preparation, handling of equipment and data analysis. Publisher Copyright: © 2025 The Author(s)
dc.description.abstractThe use of conventional plastics in our modern society presents many environmental problems, both due to the accumulation of waste in ecosystems and CO2 emissions. Polyhydroxyalkanoates (PHAs), such as the co-polymer polyhydroxy(butyrate-co-valerate) (PHBV), could serve as an alternative due to their biodegradability, biocompatibility and production from renewable raw materials. However, the downstream processing of PHBV often relies on organic solvents and harsh process conditions. This study presents a novel optimized downstream process for the extraction and purification of PHBV from mixed microbial cell (MMC) biomass, as well as recycling of the solvents used in the process. For this purpose, hydrophobic natural deep eutectic solvents (NADESs) based on the terpenes thymol and menthol were prepared, and their Hansen solubility parameters (HSPs) as well as physicochemical characteristics such as viscosity and relative polarity were analyzed, before optimization of the process was carried out. Using NADESs has several advantages over traditional solvents and extraction methods due to their low toxicity, biodegradability and tunability for different applications. After screening, the most effective NADES proved to be thymol and decanoic acid in a 1:1 M ratio, while methanol was used as an anti-solvent in a 1:3 volumetric ratio. The use of these solvents for the extraction of PHBV was then optimized and a final PHBV yield of ≈ 85 wt% and purity of ≈ 92 wt% was achieved. Furthermore, both the NADES and the methanol were recycled, closing the process loop. These results were promising, as yields were comparable to those of organic solvents. Moreover, analysis of HSPs and physicochemical characteristics could help to streamline the identification process of worthwhile NADESs solvents for use in extraction.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent6572223
dc.identifier.doi10.1016/j.cej.2025.161741
dc.identifier.issn1385-8947
dc.identifier.otherPURE: 114700281
dc.identifier.otherPURE UUID: 3d3f8049-fa57-4d4d-b3b9-a8e53263f7d4
dc.identifier.otherScopus: 105000985860
dc.identifier.otherWOS: 001457538900001
dc.identifier.otherORCID: /0000-0001-8714-3781/work/188370700
dc.identifier.otherORCID: /0000-0002-7784-0265/work/188370849
dc.identifier.urihttp://hdl.handle.net/10362/185417
dc.identifier.urlhttps://www.scopus.com/pages/publications/105000985860
dc.identifier.urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=nova_api&SrcAuth=WosAPI&KeyUT=WOS:001457538900001&DestLinkType=FullRecord&DestApp=WOS_CPL
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGeneral Chemistry
dc.subjectEnvironmental Chemistry
dc.subjectGeneral Chemical Engineering
dc.subjectIndustrial and Manufacturing Engineering
dc.subjectSDG 7 - Affordable and Clean Energy
dc.subjectSDG 12 - Responsible Consumption and Production
dc.titleZero-waste extraction of polyhydroxy(butyrate-co-valerate) (PHBV) from mixed cultures using natural deep eutectic solvents (NADES)en
dc.title.subtitleUnlocking the roles of molecular interactions, polarity, and viscosityen
dc.typejournal article
degois.publication.firstPage1
degois.publication.lastPage15
degois.publication.titleChemical Engineering Journal
degois.publication.volume511
dspace.entity.typePublication
rcaap.rightsopenAccess

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
Didion_Y._P._et_al._2025_..pdf
Tamanho:
6.27 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

Home collection (FCT)