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Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification

2023-05-20Artigo científico Acesso aberto
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dc.contributor.authorCoelho, Beatriz J.
dc.contributor.authorNeto, Joana P.
dc.contributor.authorSieira, Bárbara
dc.contributor.authorMoura, André T.
dc.contributor.authorFortunato, Elvira
dc.contributor.authorMartins, Rodrigo
dc.contributor.authorBaptista, Pedro V.
dc.contributor.authorIgreja, Rui
dc.contributor.authorÁguas, Hugo
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.institutionCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionUNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2023-07-04T22:17:54Z
dc.date.available2023-07-04T22:17:54Z
dc.date.issued2023-05-20
dc.descriptionPublisher Copyright: © 2023 by the authors.
dc.description.abstractMicrofluidic-based platforms have become a hallmark for chemical and biological assays, empowering micro- and nano-reaction vessels. The fusion of microfluidic technologies (digital microfluidics, continuous-flow microfluidics, and droplet microfluidics, just to name a few) presents great potential for overcoming the inherent limitations of each approach, while also elevating their respective strengths. This work exploits the combination of digital microfluidics (DMF) and droplet microfluidics (DrMF) on a single substrate, where DMF enables droplet mixing and further acts as a controlled liquid supplier for a high-throughput nano-liter droplet generator. Droplet generation is performed at a flow-focusing region, operating on dual pressure: negative pressure applied to the aqueous phase and positive pressure applied to the oil phase. We evaluate the droplets produced with our hybrid DMF–DrMF devices in terms of droplet volume, speed, and production frequency and further compare them with standalone DrMF devices. Both types of devices enable customizable droplet production (various volumes and circulation speeds), yet hybrid DMF–DrMF devices yield more controlled droplet production while achieving throughputs that are similar to standalone DrMF devices. These hybrid devices enable the production of up to four droplets per second, which reach a maximum circulation speed close to 1540 µm/s and volumes as low as 0.5 nL.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent2653422
dc.identifier.doi10.3390/s23104927
dc.identifier.issn1424-8220
dc.identifier.otherPURE: 65211841
dc.identifier.otherPURE UUID: 0fa35e7f-22ac-4b82-aef3-80a60cf2fe1a
dc.identifier.otherScopus: 85160424824
dc.identifier.otherWOS: 000998238000001
dc.identifier.otherORCID: /0000-0001-5255-7095/work/151383885
dc.identifier.otherORCID: /0000-0002-4202-7047/work/151403296
dc.identifier.otherORCID: /0000-0001-7350-649X/work/151423307
dc.identifier.otherORCID: /0000-0002-6608-3422/work/151426931
dc.identifier.urihttp://hdl.handle.net/10362/154845
dc.identifier.urlhttps://www.scopus.com/pages/publications/85160424824
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0037%2F2020/PT
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationIntegrated Microfluidic Device for cancer detection from free circulating DNA using Digital PCR
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationNew Generation of Digital Microfluidics for Nucleic Acid Amplification
dc.relationNew Generation of Digital Microfluidics for Nucleic Acid Amplification
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F132904%2F2017/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/COVID%2FBD%2F152453%2F2022/PT
dc.subjectdigital microfluidics
dc.subjectdroplet microfluidics
dc.subjectflow focusing
dc.subjectnegative pressure
dc.subjectAnalytical Chemistry
dc.subjectInformation Systems
dc.subjectAtomic and Molecular Physics, and Optics
dc.subjectBiochemistry
dc.subjectInstrumentation
dc.subjectElectrical and Electronic Engineering
dc.titleHybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplificationen
dc.title.subtitleDesign, Fabrication and Characterizationen
dc.typejournal article
degois.publication.issue10
degois.publication.titleSensors
degois.publication.volume23
dspace.entity.typePublication
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oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleIntegrated Microfluidic Device for cancer detection from free circulating DNA using Digital PCR
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleNew Generation of Digital Microfluidics for Nucleic Acid Amplification
oaire.awardTitleNew Generation of Digital Microfluidics for Nucleic Acid Amplification
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F132904%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/COVID%2FBD%2F152453%2F2022/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
oaire.fundingStream6817 - DCRRNI ID
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