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Heterologous Expression of Immature Forms of Human Islet Amyloid Polypeptide in Yeast Triggers Intracellular Aggregation and Cytotoxicity

2020-09-03Artigo científico Acesso aberto
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dc.contributor.authorRaimundo, Ana F.
dc.contributor.authorFerreira, Sofia
dc.contributor.authorFarrim, Maria I.
dc.contributor.authorSantos, Cláudia N.
dc.contributor.authorMenezes, Regina
dc.contributor.institutionCentro de Estudos de Doenças Crónicas (CEDOC)
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.pblFrontiers Research Foundation
dc.date.accessioned2020-10-13T22:43:57Z
dc.date.available2020-10-13T22:43:57Z
dc.date.issued2020-09-03
dc.descriptionFunding: iNOVA4Health – UID/Multi/04462/2019, a program financially supported by Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência, through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is acknowledged. Funding from INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged. This study was also supported by FCT via PTDC/BIA-MOL31104/2017, UID/Multi/04462/2013- SubProj iNOVA4Health 44, and UID/Multi/04462/2019-SubProj iNOVA4Health C44 to RM, PD/BD/135504/2018 to AR. Sociedade Portuguesa de Diabetologia for the Nuno CasteloBranco Prize – 2016 attributed to RM is also acknowledged.
dc.description.abstractDiabetes is a major public health issue that has attained alarming levels worldwide. Pancreatic aggregates of human islet amyloid polypeptide (IAPP) represent a major histopathological hallmark of type 2 diabetes. IAPP is expressed in β-cells as pre-pro-IAPP (ppIAPP) that is first processed to pro-IAPP (pIAPP) and finally to its mature form (matIAPP), being released upon glucose stimulation together with insulin. Impairment and overload of the IAPP processing machinery seem to be associated with the accumulation of immature IAPP species and the formation of toxic intracellular oligomers, which have been associated with β-cell dyshomeostasis and apoptosis. Nevertheless, the pathological importance of these immature IAPP forms for the assembly and cytotoxicity of these oligomers is not completely understood. Here, we describe the generation and characterization of unprecedented Saccharomyces cerevisiae models recapitulating IAPP intracellular oligomerization. Expression of green fluorescent protein (GFP) fusions of human ppIAPP, pIAPP, and matIAPP proved to be toxic in yeast cells at different extents, with ppIAPP exerting the most deleterious effect on yeast growth and cell viability. Although expression of all IAPP constructs induced the formation of intracellular aggregates in yeast cells, our data point out the accumulation of insoluble oligomeric species enriched in immature ppIAPP as the trigger of the high toxicity mediated by this construct in cells expressing ppIAPP-GFP. In addition, MS/MS analysis indicated that oligomeric species found in the ppIAPP-GFP lysates contain the N-terminal sequence of the propeptide fused to GFP. These models represent powerful tools for future research focused on the relevance of immature forms in IAPP-induced toxicity. Furthermore, they are extremely useful in high-throughput screenings for genetic and chemical modulators of IAPP aggregation.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1997761
dc.identifier.doi10.3389/fmicb.2020.02035
dc.identifier.issn1664-302X
dc.identifier.otherPURE: 20226289
dc.identifier.otherPURE UUID: 00249557-1929-4a8c-809f-0c696d445e37
dc.identifier.otherScopus: 85091236682
dc.identifier.otherORCID: /0000-0002-5809-1924/work/81924680
dc.identifier.otherPubMed: 33013747
dc.identifier.otherWOS: 000570522500001
dc.identifier.urihttp://hdl.handle.net/10362/105562
dc.identifier.urlhttps://www.scopus.com/pages/publications/85091236682
dc.language.isoeng
dc.peerreviewedyes
dc.subjectimmature islet amyloid polypeptide
dc.subjectislet amyloid polypeptide
dc.subjectislet amyloid polypeptide-induced toxicity
dc.subjectoligomerization
dc.subjectprotein aggregation
dc.subjectyeast model
dc.subjectMicrobiology
dc.subjectMicrobiology (medical)
dc.subjectSDG 3 - Good Health and Well-being
dc.titleHeterologous Expression of Immature Forms of Human Islet Amyloid Polypeptide in Yeast Triggers Intracellular Aggregation and Cytotoxicityen
dc.typejournal article
degois.publication.titleFrontiers in Microbiology
degois.publication.volume11
dspace.entity.typePublication
rcaap.rightsopenAccess

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