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A Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equina

2024-03-21Artigo científico Acesso aberto
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dc.contributor.authorAlcaide, Maria
dc.contributor.authorMoutinho Cabral, Inês
dc.contributor.authorCarvalho, Lara
dc.contributor.authorMendes, Vera M.
dc.contributor.authorAlves de Matos, António P.
dc.contributor.authorManadas, Bruno
dc.contributor.authorSaúde, Leonor
dc.contributor.authorD’Ambrosio, Mariaelena
dc.contributor.authorCosta, Pedro M.
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2024-04-17T01:24:40Z
dc.date.available2024-04-17T01:24:40Z
dc.date.issued2024-03-21
dc.descriptionFundo Azul co-financed project MARVEN (FA_05_2017_007). The Portuguese Foundation for Science and Technology (FCT), i.p., is acknowledged for funding the projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences-UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy-i4HB; UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020, and the National Mass Spectrometry Network (RNEM) [POCI-01-0145-FEDER-402-022125 Ref. ROTEIRO/0028/2013). IMC also acknowledges FCT for the Ph.D. grant ref. 2022.11150.BD.
dc.description.abstractThe current study investigates the venom-delivery system of green and red morphotypes of the sea anemone Actinia equina to disclose its potential as a source of bioactive compounds. We compared the two morphotypes using electron and optical microscopy, proteomics, and toxicity assessment on zebrafish embryos. Specialized venom-injecting cells (nematocysts) are equally distributed and found in the tentacles of both varieties. Proteomics revealed proteins of interest in both red and green Actinia, yielding the three most abundant Gene Ontology (GO) terms related to the biological processes “proteolysis”, “hemolysis in another organism” and “lipid catabolic process”. Neurotoxins and cytolytic toxins similar to known cnidarian toxins like PsTX-60A and AvTX-60A, for instance, were identified in both types. Extracts from green and red anemones were toxic to zebrafish embryos, with green anemone venom appearing to be more potent. The findings highlight the presence of proteinaceous toxins in A. equina and the potential for different varieties to possess distinct bioactive compounds. Notably, pore-forming toxins are suggested for molecular probes and immunotoxins, making them valuable assets for potential biotechnological and biomedical purposes.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent17
dc.format.extent6897444
dc.identifier.doi10.3390/ani14060981
dc.identifier.issn2076-2615
dc.identifier.otherPURE: 87469604
dc.identifier.otherPURE UUID: 50e32ecf-6901-497b-a7d1-edd0714725a8
dc.identifier.otherScopus: 85188816379
dc.identifier.otherWOS: 001191879000001
dc.identifier.otherPubMed: 38540078
dc.identifier.otherPubMedCentral: PMC10967595
dc.identifier.urihttp://hdl.handle.net/10362/166304
dc.identifier.urlhttps://www.scopus.com/pages/publications/85188816379
dc.language.isoeng
dc.peerreviewedyes
dc.relationPublisher Copyright: © 2024 by the authors. Funding: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology - Associate Laboratory
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0058%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT//2022.11150.BD/PT
dc.subjectbioinformatics
dc.subjectcnidaria
dc.subjectelectron microscopy
dc.subjecthistology
dc.subjectmarine bioprospecting
dc.subjectproteomics
dc.subjecttoxicity
dc.subjecttoxins
dc.subjectAnimal Science and Zoology
dc.subjectGeneral Veterinary
dc.subjectSDG 14 - Life Below Water
dc.titleA Comparative Analysis of the Venom System between Two Morphotypes of the Sea Anemone Actinia equinaen
dc.typejournal article
degois.publication.issue6
degois.publication.titleAnimals
degois.publication.volume14
dspace.entity.typePublication
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumberUIDB/04539/2020
oaire.awardNumberUIDP/04539/2020
oaire.awardNumberLA/P/0058/2020
oaire.awardNumber2022.11150.BD
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology - Associate Laboratory
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0058%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2022.11150.BD/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
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project.funder.identifierhttp://doi.org/10.13039/501100001871
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