Orientador(es)
Resumo(s)
The assessment of marine microbial biodiversity is crucial for determining either the environmental status (ecological or biodiversity research) or as a first step in the biodiscovery process (biotechnological research). Regardless of the research purpose, the first step in a biodiversity assessment is sampling, which can range from ad hoc sampling expeditions to long-term monitoring campaigns. In spite of its demands for funds, infrastructure, expertise, equipment, and personnel, sampling is often not adequately planned. This results in increased likelihood for biased sampling, which can lead to misinterpretation of results, omission of valuable specimens and an unrepresentative collection of stored samples, all particularly important for the assessment of microbial biodiversity. For these reasons, we are proposing a conceptual framework to assist in better preparation of sampling, consisting of pre-sampling, sampling and post sampling steps. The manuscript guides the reader through all the necessary steps, regardless of the sampling habitat (from water column to sediment), the sampling techniques and the preservation and storage approaches including culture and biorepositories. Such a harmonized approach can be of benefit for (i) researchers in the field of ecology/biotechnology, (ii) industrial companies requiring information on the providers and availability of data, and (iii) governance structures and funders, in the light of open science principles.
Descrição
Publisher Copyright: Copyright © 2025 Ljubešić, Gligora Udovič, Overlingė, Grgurević, Akgül, Bacu, Díaz-Marrero, Drakulović, Fazi, Gaudêncio, Kolda, Novoveska, Safarik, Sousa, Thomas, Reddy, Varese, Vasquez, Makovec and Rotter.
Palavras-chave
biodiscovery biorepositories cultures marine microbial diversity sample preservation and storage sampling protocol Oceanography Global and Planetary Change Aquatic Science Water Science and Technology Environmental Science (miscellaneous) Ocean Engineering SDG 14 - Life Below Water
