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Genomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambique

2023Artigo científico Acesso aberto
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dc.contributor.authorManjate, Filomena
dc.contributor.authorJoão, Eva D.
dc.contributor.authorMwangi, Peter
dc.contributor.authorChirinda, Percina
dc.contributor.authorMogotsi, Milton
dc.contributor.authorMessa, Augusto
dc.contributor.authorGarrine, Marcelino
dc.contributor.authorVubil, Delfino
dc.contributor.authorNobela, Nélio
dc.contributor.authorNhampossa, Tacilta
dc.contributor.authorAcácio, Sozinho
dc.contributor.authorTate, Jacqueline E.
dc.contributor.authorParashar, Umesh
dc.contributor.authorWeldegebriel, Goitom
dc.contributor.authorMwenda, Jason M.
dc.contributor.authorAlonso, Pedro L.
dc.contributor.authorCunha, Celso
dc.contributor.authorNyaga, Martin
dc.contributor.authorMandomando, Inácio
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.pblFrontiers Research Foundation
dc.date.accessioned2023-07-06T22:14:55Z
dc.date.available2023-07-06T22:14:55Z
dc.date.issued2023
dc.description870–15 SC; the United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investigação (FNI), Moçambique, grant number 245-INV, funded the surveillance of rotavirus and other enteropathogens in children less than 5 years of age in Manhiça, in the context of the implementation of the diarrhoeal disease surveillance platform. The Child Health and Mortality Prevention program (Surveillance), CHAMPS funded by the Bill & Melinda Gates Foundation under Grant OPP1126780, the Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, Portugal, the Next Generation Sequencing Unit, and the Division of Virology, Faculty of Health Sciences, University of the Free State, South Africa, supported the whole genome analysis costs. The Calouste Gulbenkian Foundation finances Filomena Manjate’s Ph.D. studies under grant number 234066. CISM receives core funding from the Mozambican government and the “Agencia Española de Cooperacion Internacional para el Desarollo (AECID).” Publisher Copyright: Copyright © 2023 Manjate, João, Mwangi, Chirinda, Mogotsi, Messa, Garrine, Vubil, Nobela, Nhampossa, Acácio, Tate, Parashar, Weldegebriel, Mwenda, Alonso, Cunha, Nyaga and Mandomando.
dc.description.abstractMozambique introduced the rotavirus vaccine (Rotarix®; GlaxoSmithKline Biologicals, Rixensart, Belgium) in 2015, and since then, the Centro de Investigação em Saúde de Manhiça has been monitoring its impact on rotavirus-associated diarrhea and the trend of circulating strains, where G3P[8] was reported as the predominant strain after the vaccine introduction. Genotype G3 is among the most commonly detected Rotavirus strains in humans and animals, and herein, we report on the whole genome constellation of G3P[8] detected in two children (aged 18 months old) hospitalized with moderate-to-severe diarrhea at the Manhiça District Hospital. The two strains had a typical Wa-like genome constellation (I1-R1-C1-M1-A1-N1-T1-E1-H1) and shared 100% nucleotide (nt) and amino acid (aa) identities in 10 gene segments, except for VP6. Phylogenetic analysis demonstrated that genome segments encoding VP7, VP6, VP1, NSP3, and NSP4 of the two strains clustered most closely with porcine, bovine, and equine strains with identities ranging from 86.9–99.9% nt and 97.2–100% aa. Moreover, they consistently formed distinct clusters with some G1P[8], G3P[8], G9P[8], G12P[6], and G12P[8] strains circulating from 2012 to 2019 in Africa (Mozambique, Kenya, Rwanda, and Malawi) and Asia (Japan, China, and India) in genome segments encoding six proteins (VP2, VP3, NSP1-NSP2, NSP5/6). The identification of segments exhibiting the closest relationships with animal strains shows significant diversity of rotavirus and suggests the possible occurrence of reassortment events between human and animal strains. This demonstrates the importance of applying next-generation sequencing to monitor and understand the evolutionary changes of strains and evaluate the impact of vaccines on strain diversity.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent12
dc.format.extent1740890
dc.identifier.doi10.3389/fmicb.2023.1193094
dc.identifier.issn1664-302X
dc.identifier.otherPURE: 65013190
dc.identifier.otherPURE UUID: 73237972-b275-4651-85de-055342e2bcc0
dc.identifier.otherScopus: 85162221553
dc.identifier.otherWOS: WOS:001008041700001
dc.identifier.otherPubMed: 37342557
dc.identifier.otherPubMedCentral: PMC10277737
dc.identifier.otherORCID: /0000-0003-4745-3819/work/151419267
dc.identifier.urihttp://hdl.handle.net/10362/154923
dc.identifier.urlhttps://www.scopus.com/pages/publications/85162221553
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: The GAVI, the vaccine alliance via the Centers for Disease Control and Prevention (CDC), Atlanta, and the World Health Organization, Regional Office for Africa (WHO/AFRO), grant number MOA
dc.subjectchildren <5 years
dc.subjecthuman G3P[8] strains
dc.subjectmoderate-to-severe diarrhea
dc.subjectreassortments
dc.subjectrotavirus group A
dc.subjectwhole-genome sequencing
dc.subjectRJ101 Child Health. Child health services
dc.subjectQR355 Virology
dc.subjectMicrobiology
dc.subjectMicrobiology (medical)
dc.subjectGastroenterology
dc.subjectInfectious Diseases
dc.subjectPediatrics, Perinatology, and Child Health
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectVirology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleGenomic characterization of the rotavirus G3P[8] strain in vaccinated children, reveals possible reassortment events between human and animal strains in Manhiça District, Mozambiqueen
dc.typejournal article
degois.publication.titleFrontiers in Microbiology
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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