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Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts

2023-03-04Artigo científico Acesso aberto
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dc.contributor.authorMalmir, Maryam
dc.contributor.authorLima, Katelene
dc.contributor.authorPóvoas Camões, Sérgio
dc.contributor.authorManageiro, Vera
dc.contributor.authorDuarte, Maria Paula
dc.contributor.authorPaiva Miranda, Joana
dc.contributor.authorSerrano, Rita
dc.contributor.authorMoreira da Silva, Isabel
dc.contributor.authorSilva Lima, Beatriz
dc.contributor.authorCaniça, Manuela
dc.contributor.authorSilva, Olga
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionMEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2023-09-27T22:26:44Z
dc.date.available2023-09-27T22:26:44Z
dc.date.issued2023-03-04
dc.descriptionPublisher Copyright: © 2023 by the authors.
dc.description.abstractThe leaves of Asphodelus bento-rainhae subsp. bento-rainhae, an endemic Portuguese species, and Asphodelus macrocarpus subsp. macrocarpus have been used as food, and traditionally as medicine, for treating ulcers, urinary tract, and inflammatory disorders. The present study aims to establish the phytochemical profile of the main secondary metabolites, together with the antimicrobial, antioxidant and toxicity assessments of both Asphodelus leaf 70% ethanol extracts. Phytochemical screenings were conducted by the TLC and LC-UV/DAD-ESI/MS chromatographic technique, and quantification of the leading chemical classes was performed by spectrophotometric methods. Liquid-liquid partitions of crude extracts were obtained using ethyl ether, ethyl acetate, and water. For in vitro evaluations of antimicrobial activity, the broth microdilution method, and for the antioxidant activity, the FRAP and DPPH methods were used. Genotoxicity and cytotoxicity were assessed by Ames and MTT tests, respectively. Twelve known compounds including neochlorogenic acid, chlorogenic acid, caffeic acid, isoorientin, p-coumaric acid, isovitexin, ferulic acid, luteolin, aloe-emodin, diosmetin, chrysophanol, and β-sitosterol were identified as the main marker compounds, and terpenoids and condensed tannins were found to be the major class of secondary metabolites of both medicinal plants. The ethyl ether fractions demonstrated the highest antibacterial activity against all the Gram-positive microorganisms, (MIC value of 62 to 1000 µg/mL), with aloe-emodin as one of the main marker compounds highly active against Staphylococcus epidermidis (MIC value of 0.8 to 1.6 µg/mL). Ethyl acetate fractions exhibited the highest antioxidant activity (IC50 of 800 to 1200 µg/mL, respectively). No cytotoxicity (up to 1000 µg/mL) or genotoxicity/mutagenicity (up to 5 mg/plate, with/without metabolic activation) were detected. The obtained results contribute to the knowledge of the value and safety of the studied species as herbal medicines.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent18
dc.format.extent2152942
dc.identifier.doi10.3390/molecules28052372
dc.identifier.issn1420-3049
dc.identifier.otherPURE: 72622156
dc.identifier.otherPURE UUID: e4c71fb2-e451-4120-af60-a5fdb50e1e20
dc.identifier.otherScopus: 85149849228
dc.identifier.otherWOS: 000948198900001
dc.identifier.otherPubMed: 36903618
dc.identifier.otherPubMedCentral: PMC10005749
dc.identifier.urihttp://hdl.handle.net/10362/158393
dc.identifier.urlhttps://www.scopus.com/pages/publications/85149849228
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
dc.relationResearch Institute for Medicines
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationDiscovery of new antimicrobial drugs active against resistant pathogen agents: The case study of Portuguese Asphodelus species
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F125310%2F2016/PT
dc.subjectAloe-emodin
dc.subjectantimicrobial
dc.subjectantioxidant
dc.subjectAsphodelus
dc.subjectchemical profile
dc.subjectherbal medicines
dc.subjectpreclinical safety
dc.subjectStaphylococcus epidermidis
dc.subjectAnalytical Chemistry
dc.subjectChemistry (miscellaneous)
dc.subjectMolecular Medicine
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.titleIdentification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extractsen
dc.typejournal article
degois.publication.issue5
degois.publication.titleMolecules
degois.publication.volume28
dspace.entity.typePublication
oaire.awardNumberUIDB/04138/2020
oaire.awardNumberUIDP/04077/2020
oaire.awardNumberUIDB/04077/2020
oaire.awardNumberSFRH/BD/125310/2016
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleDiscovery of new antimicrobial drugs active against resistant pathogen agents: The case study of Portuguese Asphodelus species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F125310%2F2016/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication0538216a-abc4-47bc-a64a-9938dd65376f
relation.isProjectOfPublication61d74fe4-0039-4426-95b9-e3dda527fe0b
relation.isProjectOfPublication95687852-8680-45e0-bc50-61923a3aa0a0
relation.isProjectOfPublicatione96ff671-6415-41dc-858a-81f2f62514ef
relation.isProjectOfPublication.latestForDiscovery61d74fe4-0039-4426-95b9-e3dda527fe0b

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