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Localization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillus

2021-05-15Artigo científico Acesso aberto
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dc.contributor.authorD’Ambrosio, Mariaelena
dc.contributor.authorGonçalves, Cátia
dc.contributor.authorCalmão, Mariana
dc.contributor.authorRodrigues, Maria
dc.contributor.authorCosta, Pedro M.
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-04-12T22:48:10Z
dc.date.available2022-04-12T22:48:10Z
dc.date.issued2021-05-15
dc.descriptionFA_05_2017_007 UID/Multi/04378/2020
dc.description.abstractMarine biodiversity has been yielding promising novel bioproducts from venomous animals. Despite the auspices of conotoxins, which originated the paradigmatic painkiller Prialt, the biotechnological potential of gastropod venoms remains to be explored. Marine bioprospecting is expanding towards temperate species like the dogwhelk Nucella lapillus, which is suspected to secrete immobilizing agents through its salivary glands with a relaxing effect on the musculature of its preferential prey, Mytilus sp. This work focused on detecting, localizing, and testing the bioreactivity of cysteine-rich proteins and peptides, whose presence is a signature of animal venoms and poisons. The highest content of thiols was found in crude protein extracts from the digestive gland, which is associated with digestion, followed by the peribuccal mass, where the salivary glands are located. Conversely, the foot and siphon (which the gastropod uses for feeding) are not the main organs involved in toxin secretion. Ex vivo bioassays with Mytilus gill tissue disclosed the differential bioreactivity of crude protein extracts. Secretions from the digestive gland and peribuccal mass caused the most significant molecular damage, with evidence for the induction of apoptosis. These early findings indicate that salivary glands are a promising target for the extraction and characterization of bioactive cysteine-rich proteinaceous toxins from the species.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent9362149
dc.identifier.doi10.3390/md19050276
dc.identifier.issn1660-3397
dc.identifier.otherPURE: 42960187
dc.identifier.otherPURE UUID: 55349a52-7a51-470d-aaa5-4ffac1e8c729
dc.identifier.otherScopus: 85107454949
dc.identifier.otherPubMed: 34063509
dc.identifier.otherPubMedCentral: PMC8156292
dc.identifier.otherWOS: 000654430400001
dc.identifier.urihttp://hdl.handle.net/10362/136329
dc.identifier.urlhttps://www.scopus.com/pages/publications/85107454949
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTA-BTA%2F28650%2F2017/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F144914%2F2019/PT
dc.relationColeoid venoms: Predicting cephalotoxin function and biotechnological applications from ecological and evolutionary traits
dc.subjectBioreactives
dc.subjectDogwhelk
dc.subjectGastropoda
dc.subjectMarine biotechnology
dc.subjectThiols
dc.subjectToxicity
dc.subjectDrug Discovery
dc.subjectSDG 14 - Life Below Water
dc.titleLocalization and bioreactivity of cysteine-rich secretions in the marine gastropod nucella lapillusen
dc.typejournal article
degois.publication.issue5
degois.publication.titleMarine Drugs
degois.publication.volume19
dspace.entity.typePublication
oaire.awardNumberPTDC/BTA-BTA/28650/2017
oaire.awardNumberSFRH/BD/144914/2019
oaire.awardTitleColeoid venoms: Predicting cephalotoxin function and biotechnological applications from ecological and evolutionary traits
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FBTA-BTA%2F28650%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F144914%2F2019/PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication78ae6b76-01f0-4da9-a113-b6333b88dd66
relation.isProjectOfPublication90ccba4c-8afb-4404-87fd-2be1b81e7522
relation.isProjectOfPublication.latestForDiscovery90ccba4c-8afb-4404-87fd-2be1b81e7522

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