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Diversity of Pneumocystis jirovecii Across Europe:

2017-08-01Artigo científico Acesso aberto
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dc.contributor.authorAlanio, Alexandre
dc.contributor.authorGits-Muselli, Maud
dc.contributor.authorGuigue, Nicolas
dc.contributor.authorDesnos-Ollivier, Marie
dc.contributor.authorCalderon, Enrique J.
dc.contributor.authorDi Cave, David
dc.contributor.authorDupont, Damien
dc.contributor.authorHamprecht, Axel
dc.contributor.authorHauser, Philippe M.
dc.contributor.authorHelweg-Larsen, Jannik
dc.contributor.authorKicia, Marta
dc.contributor.authorLagrou, Katrien
dc.contributor.authorLengerova, Martina
dc.contributor.authorMatos, Olga
dc.contributor.authorMelchers, Willem J.G.
dc.contributor.authorMorio, Florent
dc.contributor.authorNevez, Gilles
dc.contributor.authorTotet, Anne
dc.contributor.authorWhite, Lewis P.
dc.contributor.authorBretagne, Stéphane
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.pblElsevier
dc.date.accessioned2018-05-15T22:06:14Z
dc.date.available2018-05-15T22:06:14Z
dc.date.issued2017-08-01
dc.description.abstractPneumocystis jirovecii is an airborne human-specific ascomycetous fungus responsible for Pneumocystis pneumonia (PCP) in immunocompromised patients, affecting > 500,000 patients per year (www.gaffi.org). The understanding of its epidemiology is limited by the lack of standardised culture. Recent genotyping data suggests a limited genetic diversity of P. jirovecii. The objective of the study was to assess the diversity of P. jirovecii across European hospitals and analyse P. jirovecii diversity in respect to clinical data obtained from the patients. Genotyping was performed using six already validated short tandem repeat (STR) markers on 249 samples (median: 17 per centre interquartile range [11 − 20]) from PCP patients of 16 European centres. Mixtures of STR markers (i.e., ≥ 2 alleles for ≥ 1 locus) were detected in 67.6% (interquartile range [61.4; 76.5]) of the samples. Mixture was significantly associated with the underlying disease of the patient, with an increased proportion in HIV patients (78.3%) and a decreased proportion in renal transplant recipients (33.3%) (p < 0.001). The distribution of the alleles was significantly different (p < 0.001) according to the centres in three out of six markers. In analysable samples, 201 combinations were observed corresponding to 137 genotypes: 116 genotypes were country-specific; 12 in two; six in three; and two in four and one in five countries. Nine genotypes were recorded more than once in a given country. Genotype 123 (Gt123) was significantly associated with France (14/15, p < 0.001) and Gt16 with Belgium (5/5, p < 0.001). More specifically, Gt123 was observed mainly in France (14/15/16 patients) and in renal transplant patient (13/15). Our study showed the wide population diversity across Europe, with evidence of local clusters of patients harbouring a given genotype. These data suggest a specific association between genotype and underlying disease, with evidence of a different natural history of PCP in HIV patients and renal transplant recipients.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent9
dc.format.extent1006434
dc.identifier.doi10.1016/j.ebiom.2017.06.027
dc.identifier.issn2352-3964
dc.identifier.otherPURE: 3182179
dc.identifier.otherPURE UUID: 45b2bf82-7883-4fb6-beb2-78b3e4803bd5
dc.identifier.otherScopus: 85021876565
dc.identifier.otherWOS: 000410738600020
dc.identifier.otherPubMed: 28705464
dc.identifier.otherPubMedCentral: PMC5552205
dc.identifier.otherORCID: /0000-0001-5793-7716/work/45845543
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85021876565&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/85021876565
dc.language.isoeng
dc.peerreviewedyes
dc.subjectEurope
dc.subjectGenotyping
dc.subjectMicrosatellites
dc.subjectMixed infection
dc.subjectMLS typing
dc.subjectPneumocystis jirovecii
dc.subjectTransmission
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectInfectious Diseases
dc.subjectEpidemiology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleDiversity of Pneumocystis jirovecii Across Europe:en
dc.title.subtitleA Multicentre Observational Studyen
dc.typejournal article
degois.publication.firstPage155
degois.publication.lastPage163
degois.publication.titleEBioMedicine
degois.publication.volume22
dspace.entity.typePublication
rcaap.rightsopenAccess

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