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Tailoring the properties of rhamnose-rich exopolysaccharides from cheese whey via continuous fed-batch fermentation for active packaging applications

2026-06Artigo científico Acesso aberto
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dc.contributor.authorAzabou, Samia
dc.contributor.authorJoulak, Ichrak
dc.contributor.authorConcórdio-Reis, Patrícia
dc.contributor.authorTorres, Cristiana A. V.
dc.contributor.authorSevrin, Chantal
dc.contributor.authorGrandfils, Christian
dc.contributor.authorAttia, Hamadi
dc.contributor.authorGharsallaoui, Adem
dc.contributor.authorFreitas, Filomena
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.pblElsevier
dc.date.accessioned2026-05-25T10:20:01Z
dc.date.available2026-05-25T10:20:01Z
dc.date.issued2026-06
dc.descriptionPublisher Copyright: © 2026 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license. http://creativecommons.org/licenses/by/4.0/
dc.description.abstractThis study explores the sustainable bioconversion of cheese whey (CW) into high-value exopolysaccharides (EPS) by Halomonas caseinilytica K1 through the optimization of bioreactor operation modes. Batch, fed-batch with pulse feeding, and fed-batch with continuous feeding were compared to evaluate their impact on cell growth, EPS production, and polymer characteristics. The continuous fed-batch strategy markedly outperformed the other modes, achieving the highest biomass concentration (89.95 g/L) and EPS titer (11.67 g/L), corresponding to 2.12- and 5.16-fold increases relative to pulse-fed and batch cultures, respectively. This cultivation approach significantly influenced EPS structure, yielding a rhamnose-enriched polymer with a high molecular weight of 2.25 × 10⁵ Da, corresponding to a fourfold increase. The optimized EPS (EPS-K1-B3) was subsequently applied to fabricate biodegradable antimicrobial films incorporating geraniol (10%). These films displayed good structural integrity and demonstrated sustained inhibitory activity against Escherichia coli and Listeria innocua over a 22-day release period. Overall, this work establishes an integrated bioprocess–structure–function framework, demonstrating how controlled fermentation conditions can tailor EPS properties for targeted applications in active food packaging while contributing to the valorization of agro-industrial waste streams.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent9
dc.format.extent1941999
dc.identifier.doi10.1016/j.carpta.2026.101128
dc.identifier.issn2666-8939
dc.identifier.otherPURE: 162793850
dc.identifier.otherPURE UUID: cafd9fda-6b3c-43f7-8bfe-b587b94b47c7
dc.identifier.otherScopus: 105034670984
dc.identifier.otherWOS: 001734382800001
dc.identifier.otherORCID: /0000-0002-9430-4640/work/215726411
dc.identifier.urihttp://hdl.handle.net/10362/203365
dc.identifier.urlhttps://www.scopus.com/pages/publications/105034670984
dc.identifier.urlhttps://www.webofscience.com/wos/woscc/full-record/WOS:001734382800001
dc.language.isoeng
dc.peerreviewedyes
dc.subjectActive packaging
dc.subjectAntimicrobial films
dc.subjectCheese whey valorization
dc.subjectExopolysaccharide
dc.subjectFed-batch fermentation
dc.subjectHalomonas caseinilytica K1
dc.subjectPolysaccharide characterization
dc.subjectBiotechnology
dc.subjectAnalytical Chemistry
dc.subjectChemistry (miscellaneous)
dc.subjectBiochemistry
dc.subjectPolymers and Plastics
dc.subjectMaterials Chemistry
dc.titleTailoring the properties of rhamnose-rich exopolysaccharides from cheese whey via continuous fed-batch fermentation for active packaging applicationsen
dc.typejournal article
degois.publication.firstPage1
degois.publication.lastPage9
degois.publication.titleCarbohydrate Polymer Technologies and Applications
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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