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Immunophenotyping of peripheral blood, lymph node, and bone marrow T lymphocytes during canine leishmaniosis and the impact of antileishmanial chemotherapy

2020-07-15Artigo científico Acesso aberto
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dc.contributor.authorSantos, Marcos Ferreira
dc.contributor.authorAlexandre-Pires, Graça
dc.contributor.authorPereira, Maria A.
dc.contributor.authorGomes, Lídia
dc.contributor.authorRodrigues, Armanda V.
dc.contributor.authorBasso, Alexandra
dc.contributor.authorReisinho, Ana
dc.contributor.authorMeireles, José
dc.contributor.authorSantos-Gomes, Gabriela M.
dc.contributor.authorPereira da Fonseca, Isabel
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblFrontiers Media
dc.date.accessioned2021-05-01T22:53:11Z
dc.date.available2021-05-01T22:53:11Z
dc.date.issued2020-07-15
dc.description.abstractDogs are a major reservoir of Leishmania infantum, etiological agent of canine leishmaniosis (CanL) a zoonotic visceral disease of worldwide concern. Therapeutic protocols based on antileishmanial drugs are commonly used to treat sick dogs and improve their clinical condition. To better understand the impact of Leishmania infection and antileishmanial drugs on the dog's immune response, this study investigates the profile of CD4+ and CD8+ T cell subsets in peripheral blood, lymph node, and bone marrow of sick dogs and after two different CanL treatments. Two CanL groups of six dogs each were treated with either miltefosine or meglumine antimoniate combined with allopurinol. Another group of 10 clinically healthy dogs was used as control. Upon diagnosis and during the following 3 months of treatment, peripheral blood, popliteal lymph node, and bone marrow mononuclear cells were collected, labeled for surface markers CD45, CD3, CD4, CD8, CD25, and intracellular nuclear factor FoxP3, and T lymphocyte subpopulations were immunophenotyped by flow cytometry. CanL dogs presented an overall increased frequency of CD8+ and CD4+CD8+ double-positive T cells in all tissues and a decreased frequency of CD4+ T cells in the blood. Furthermore, there was a higher frequency of CD8+ T cells expressing CD25+FoxP3+ in the blood and bone marrow. During treatment, these subsets recovered to levels similar to those of healthy dogs. Nevertheless, antileishmanial therapy caused an increase of CD4+CD25+FoxP3+ T cells in all tissues, associated with the decrease of CD8+CD25−FoxP3− T cell percentages. These findings may support previous studies that indicate that L. infantum manipulates the dog's immune system to avoid the development of a protective response, ensuring the parasite's survival and the conditions that allow the completion of Leishmania life cycle. Both treatments used appear to have an effect on the dog's immune response, proving to be effective in promoting the normalization of T cell subsets.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent19
dc.format.extent2553533
dc.identifier.doi10.3389/fvets.2020.00375
dc.identifier.issn2297-1769
dc.identifier.otherPURE: 26687627
dc.identifier.otherPURE UUID: 0be05370-d291-45dc-a2af-9806a576d4c3
dc.identifier.otherScopus: 85088795907
dc.identifier.otherWOS: 000556459200001
dc.identifier.otherPubMed: 32760744
dc.identifier.otherPubMedCentral: PMC7373748
dc.identifier.otherORCID: /0000-0001-9264-3887/work/125066196
dc.identifier.urihttp://hdl.handle.net/10362/116634
dc.identifier.urlhttps://www.scopus.com/pages/publications/85088795907
dc.identifier.urlhttps://www.frontiersin.org/articles/10.3389/fvets.2020.00375/full
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAntileishmanial therapy
dc.subjectBone marrow
dc.subjectCanine leishmaniosis
dc.subjectEffector T cells
dc.subjectFow cytometry
dc.subjectLymph node
dc.subjectPeripheral blood mononuclear cells
dc.subjectRegulatory T (Treg) cells
dc.subjectGeneral Veterinary
dc.subjectImmunology
dc.subjectParasitology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleImmunophenotyping of peripheral blood, lymph node, and bone marrow T lymphocytes during canine leishmaniosis and the impact of antileishmanial chemotherapyen
dc.typejournal article
degois.publication.firstPage375
degois.publication.lastPage394
degois.publication.titleFrontiers in Veterinary Science
degois.publication.volume7
dspace.entity.typePublication
rcaap.rightsopenAccess

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