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Influence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cells

2024-06Artigo científico Acesso aberto
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dc.contributor.authorSerambeque, Beatriz
dc.contributor.authorSerambeque, Beatriz
dc.contributor.authorMestre, Catarina
dc.contributor.authorCorreia-Barros, Gabriela
dc.contributor.authorBarros, Gabriela
dc.contributor.authorTeixo, Ricardo
dc.contributor.authorMarques Teixo, Ricardo Jorge
dc.contributor.authorMarto, Carlos Miguel
dc.contributor.authorMarto, Carlos Miguel
dc.contributor.authorGonçalves, Ana Cristina
dc.contributor.authorGonçalves, Ana Cristina
dc.contributor.authorCaramelo, Francisco
dc.contributor.authorCaramelo, Francisco
dc.contributor.authorSilva, Isabel
dc.contributor.authorPaiva, Artur
dc.contributor.authorPaiva, Artur
dc.contributor.authorBeck, Hans C.
dc.contributor.authorCarvalho, Ana Sofia
dc.contributor.authorAS, Carvalho
dc.contributor.authorBotelho, Maria Filomena
dc.contributor.authorBotelho, MF
dc.contributor.authorCarvalho, Maria João
dc.contributor.authorCarvalho, Maria João
dc.contributor.authorMatthiesen, Rune
dc.contributor.authorMatthiesen, Rune
dc.contributor.authorLaranjo, Mafalda
dc.contributor.authorLaranjo, Mafalda
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutioniNOVA4Health - pólo NMS
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2024-06-20T00:39:05Z
dc.date.available2024-06-20T00:39:05Z
dc.date.issued2024-06
dc.descriptionPublisher Copyright: © 2024 by the authors.
dc.description.abstractEndometrial cancer is one of the most common gynaecological malignancies. Although often diagnosed at an early stage, there is a subset of patients with recurrent and metastatic disease for whom current treatments are not effective. Cancer stem cells (CSCs) play a pivotal role in triggering tumorigenesis, disease progression, recurrence, and metastasis, as high aldehyde dehydrogenase (ALDH) activity is associated with invasiveness and chemotherapy resistance. Therefore, this study aimed to evaluate the effects of ALDH inhibition in endometrial CSCs. ECC-1 and RL95-2 cells were submitted to a sphere-forming protocol to obtain endometrial CSCs. ALDH inhibition was evaluated through ALDH activity and expression, sphere-forming capacity, self-renewal, projection area, and CD133, CD44, CD24, and P53 expression. A mass spectrometry-based proteomic study was performed to determine the proteomic profile of endometrial cancer cells upon N,N-diethylaminobenzaldehyde (DEAB). DEAB reduced ALDH activity and expression, along with a significant decrease in sphere-forming capacity and projection area, with increased CD133 expression. Additionally, DEAB modulated P53 expression. Endometrial cancer cells display a distinct proteomic profile upon DEAB, sharing 75 up-regulated and 30 down-regulated proteins. In conclusion, DEAB inhibits ALDH activity and expression, influencing endometrial CSC phenotype. Furthermore, ALDH18A1, SdhA, and UBAP2L should be explored as novel molecular targets for endometrial cancer.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2845801
dc.identifier.doi10.3390/cancers16112031
dc.identifier.issn2072-6694
dc.identifier.otherPURE: 93540217
dc.identifier.otherPURE UUID: 5d25037a-64ca-4659-ad56-c8cd5c3d6e28
dc.identifier.otherScopus: 85195805733
dc.identifier.urihttp://hdl.handle.net/10362/168824
dc.identifier.urlhttps://www.scopus.com/pages/publications/85195805733
dc.language.isoeng
dc.peerreviewedyes
dc.subjectaldehyde dehydrogenase
dc.subjectALDH inhibition
dc.subjectcancer stem cells
dc.subjectendometrial neoplasms
dc.subjectN,N-diethylaminobenzaldehyde
dc.subjectproteomics
dc.subjectstemness
dc.subjectOncology
dc.subjectCancer Research
dc.subjectSDG 3 - Good Health and Well-being
dc.titleInfluence of Aldehyde Dehydrogenase Inhibition on Stemness of Endometrial Cancer Stem Cellsen
dc.typejournal article
degois.publication.issue11
degois.publication.titleCancers
degois.publication.volume16
dspace.entity.typePublication
person.familyNameSerambeque
person.familyNameBarros
person.familyNameMarques Teixo
person.familyNameMarto
person.familyNameGonçalves
person.familyNameCaramelo
person.familyNamePaiva
person.familyNameCarvalho
person.familyNameBotelho
person.familyNameCarvalho
person.familyNameMatthiesen
person.familyNameLaranjo
person.givenNameBeatriz
person.givenNameGabriela
person.givenNameRicardo Jorge
person.givenNameCarlos Miguel
person.givenNameAna Cristina
person.givenNameFrancisco
person.givenNameArtur
person.givenNameAna Sofia
person.givenNameMaria Filomena
person.givenNameMaria João
person.givenNameRune
person.givenNameMafalda
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rcaap.rightsopenAccess
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