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Immunoproteomics of Plasmodium falciparum-infected red blood cell membrane fractions

2017-12-01Artigo científico Acesso aberto
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dc.contributor.authorCabral, Fernanda J.
dc.contributor.authorVianna, Luciana G.
dc.contributor.authorMedeiros, Marcia M.
dc.contributor.authorCarlos, Bianca Cechetto
dc.contributor.authorMartha, Rosimeire D.
dc.contributor.authorSilva, Nadia Maria
dc.contributor.authorDa Silva, Luiz Hildebrando P.
dc.contributor.authorStabeli, Rodrigo G.
dc.contributor.authorWunderlich, Gerhard
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblMemorias Do Instituto Oswaldo Cruz / Instituto Oswaldo Cruz, Ministério da Saúde
dc.date.accessioned2018-05-10T22:21:33Z
dc.date.available2018-05-10T22:21:33Z
dc.date.issued2017-12-01
dc.description.abstractBACKGROUND The surface of infected red blood cells (iRBCs) has been widely investigated because of the molecular complexity and pathogenesis mechanisms involved. Asymptomatic individuals are important in the field because they can perpetuate transmission as natural reservoirs and present a challenge for diagnosing malaria because of their low levels of circulating parasites. Recent studies of iRBC antibody recognition have shown that responses are quantitatively similar in symptomatic and asymptomatic infections, but no studies have characterised the plasmodial proteins targeted by this response. OBJECTIVES Our main objective was to identify Plasmodium falciparum proteins associated with iRBC ghosts recognised by antibodies in the sera of symptomatic and asymptomatic individuals in the Brazilian Amazon. METHODS We collected symptomatic and asymptomatic sera from patients residing in the Brazilian Amazon and P. falciparum iRBC ghosts to identify the proteins involved in natural antibody recognition by 2D-electrophoresis, western blotting, and highresolution mass spectrometry. FINDINGS 2D gel-based immunoproteome analysis using symptomatic and asymptomatic sera identified 11 proteins with at least one unique peptide, such as chaperones HSP70-1 and HSP70-x, which likely are components of the secretion machinery/PTEX translocon. PfEMP1 is involved in antigenic variation in symptomatic infections and we found putative membrane proteins whose functions are unknown. MAIN FINDINGS Our results suggest a potential role of old and new proteins, such as antigenic variation proteins, iRBC remodelling, and membrane proteins, with no assigned functions related to the immune response against P. falciparum, providing insights into the pathogenesis, erythrocyte remodelling, and secretion machinery important for alternative diagnosis and/or malaria therapy.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent7
dc.format.extent387477
dc.identifier.doi10.1590/0074-02760170041
dc.identifier.issn0074-0276
dc.identifier.otherPURE: 3764841
dc.identifier.otherPURE UUID: 39be497f-d2c4-4226-9a54-0d571656aafb
dc.identifier.otherScopus: 85037047902
dc.identifier.otherPubMed: 29211247
dc.identifier.otherScielo: 02762017001200850
dc.identifier.otherORCID: /0000-0002-5364-9087/work/177280805
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85037047902&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/85037047902
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAsymptomatic
dc.subjectPlasmodium falciparum
dc.subjectProteomics
dc.subjectSymptomatic
dc.subjectImmunology
dc.subjectParasitology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleImmunoproteomics of Plasmodium falciparum-infected red blood cell membrane fractionsen
dc.typejournal article
degois.publication.firstPage850
degois.publication.issue12
degois.publication.lastPage856
degois.publication.titleMemórias do Instituto Oswaldo Cruz
degois.publication.volume112
dspace.entity.typePublication
rcaap.rightsopenAccess

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