Logo do repositório
 
Publicação

Microarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteins

2020-01-10Recensão Acesso aberto
Ver registo simples
dc.contributor.authorCampanero-Rhodes, María Asunción
dc.contributor.authorPalma, Angelina Sá
dc.contributor.authorMenéndez, Margarita
dc.contributor.authorSolís, Dolores
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.pblFrontiers Research Foundation
dc.date.accessioned2021-05-27T23:27:15Z
dc.date.available2021-05-27T23:27:15Z
dc.date.issued2020-01-10
dc.descriptionSpanish Ministry of Economy and Competitiveness (Grant BFU2015-70052-R). Spanish Ministry of Science, Innovation, and Universities, the Spanish State Research Agency, the European Regional Development Fund (RTI2018-099985-B-I00, MCIU/AEI/FEDER, UE), the CIBER of Respiratory Diseases (CIBERES), an initiative from the Spanish Institute of Health Carlos III (ISCIII). PTDC/BIA-MIB/31730/2017. IF/00023/2012. Applied Molecular Biosciences Unit (UCIBIO) (Financed by FCT-MCTES, UID/Multi/04378/2013/2019).
dc.description.abstractBacterial surfaces are decorated with distinct carbohydrate structures that may substantially differ among species and strains. These structures can be recognized by a variety of glycan-binding proteins, playing an important role in the bacteria cross-talk with the host and invading bacteriophages, and also in the formation of bacterial microcolonies and biofilms. In recent years, different microarray approaches for exploring bacterial surface glycans and their recognition by proteins have been developed. A main advantage of the microarray format is the inherent miniaturization of the method, which allows sensitive and high-throughput analyses with very small amounts of sample. Antibody and lectin microarrays have been used for examining bacterial glycosignatures, enabling bacteria identification and differentiation among strains. In addition, microarrays incorporating bacterial carbohydrate structures have served to evaluate their recognition by diverse host/phage/bacterial glycan-binding proteins, such as lectins, effectors of the immune system, or bacterial and phagic cell wall lysins, and to identify antigenic determinants for vaccine development. The list of samples printed in the arrays includes polysaccharides, lipopoly/lipooligosaccharides, (lipo)teichoic acids, and peptidoglycans, as well as sequence-defined oligosaccharide fragments. Moreover, microarrays of cell wall fragments and entire bacterial cells have been developed, which also allow to study bacterial glycosylation patterns. In this review, examples of the different microarray platforms and applications are presented with a view to give the current state-of-the-art and future prospects in this field.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent4914689
dc.identifier.doi10.3389/fmicb.2019.02909
dc.identifier.issn1664-302X
dc.identifier.otherPURE: 16738657
dc.identifier.otherPURE UUID: 1c6707ae-823b-446a-80f4-d92d2634f92a
dc.identifier.otherScopus: 85078803680
dc.identifier.otherPubMed: 32010066
dc.identifier.otherPubMedCentral: PMC6972965
dc.identifier.otherWOS: 000509872600001
dc.identifier.urihttp://hdl.handle.net/10362/118444
dc.identifier.urlhttps://www.scopus.com/pages/publications/85078803680
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/112537/PT
dc.subjectantibodies
dc.subjectbacterial glycans
dc.subjectbacterial interactions
dc.subjectimmune system
dc.subjectlectins
dc.subjectmicroarrays
dc.subjectvaccine development
dc.subjectMicrobiology
dc.subjectMicrobiology (medical)
dc.subjectSDG 3 - Good Health and Well-being
dc.titleMicroarray Strategies for Exploring Bacterial Surface Glycans and Their Interactions With Glycan-Binding Proteinsen
dc.typereview
degois.publication.titleFrontiers in Microbiology
degois.publication.volume10
dspace.entity.typePublication
oaire.awardNumberPTDC/QUI-QUI/112537/2009
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FQUI-QUI%2F112537%2F2009/PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublication09bff4c4-1d92-4cf8-9f88-1dc4a788b45e
relation.isProjectOfPublication.latestForDiscovery09bff4c4-1d92-4cf8-9f88-1dc4a788b45e

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
fmicb_10_02909.pdf
Tamanho:
4.69 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

FCT: DQ - Artigos em revista internacional com arbitragem científica