Logo do repositório
 
Publicação

Soft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem/stromal cells (hMSCs) through mechanotransduction

2019-12-01Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorGerardo, Heloísa
dc.contributor.authorLima, Ana
dc.contributor.authorRamos, João R. D.
dc.contributor.authorCouceiro, Sofia
dc.contributor.authorCarvalho, João C. L.
dc.contributor.authorTravasso, Rui D. M.
dc.contributor.authorPires das Neves, Ricardo
dc.contributor.authorGrãos, Mário
dc.contributor.institutionFaculdade de Ciências e Tecnologia (FCT)
dc.contributor.pblNature Publishing Group
dc.date.accessioned2020-10-07T23:05:41Z
dc.date.available2020-10-07T23:05:41Z
dc.date.issued2019-12-01
dc.descriptionFundação para a Ciência e a Tecnologia) - FCT - grant FCT-UID/NEU/04539/2019. European Regional Development Fund (ERDF/FEDER) through the Operational Program Competitiveness Factors (Programa Operacional Factores de Competitividade) - COMPETE - funding through Project 'Stem cell based platforms for Regenerative and Therapeutic Medicine', Centro-07-ST24-FEDER-002008. M.G. acknowledges funding by the ERDF/FEDER through COMPETE and by national funds by FCT through grant FCOMP-01-0124-FEDER-021150 - PTDC/SAU-889 ENB/119292/2010 and grant POCI-01-0145-FEDER-029516, co-financed by the ERDF/FEDER under the framework Competitiveness and Internationalization Operational Program (Programa Operacional Competitividade e Internacionalizacao -POCI), national funds through FCT/'Ministerio da Ciencia, Tecnologia e Ensino Superior' (FCT/MCTES) through the Portuguese State Budget. Grant PTDC/SAU-ENB/113696/2009 was attributed to R.P.N. R.D.M.T. and J.C. thank the support of FEDER funds through COMPETE and by national funds by FCT under the strategic project UID/FIS/04564/2016 and under POCI-01-0145-FEDER-031743 - PTDC/BIA-CEL/31743/2017. R.D.M.T. acknowledges FCT's support through the FCT Researcher Program.
dc.description.abstractBiophysical cues influence many aspects of cell behavior. Stiffness of the extracellular matrix is probed by cells and transduced into biochemical signals through mechanotransduction protein networks, strongly influencing stem cell behavior. Cellular stemness is intimately related with mechanical properties of the cell, like intracellular contractility and stiffness, which in turn are influenced by the microenvironment. Pluripotency is associated with soft and low-contractility cells. Hence, we postulated that soft cell culture substrates, presumably inducing low cellular contractility and stiffness, increase the reprogramming efficiency of mesenchymal stem/stromal cells (MSCs) into induced pluripotent stem cells (iPSCs). We demonstrate that soft substrates (1.5 or 15 kPa polydimethylsiloxane – PDMS) caused modulation of several cellular features of MSCs into a phenotype closer to pluripotent stem cells (PSCs). MSCs cultured on soft substrates presented more relaxed nuclei, lower maturation of focal adhesions and F-actin assembling, more euchromatic and less heterochromatic nuclear DNA regions, and increased expression of pluripotency-related genes. These changes correlate with the reprogramming of MSCs, with a positive impact on the kinetics, robustness of colony formation and reprogramming efficiency. Additionally, substrate stiffness influences several phenotypic features of iPS cells and colonies, and data indicates that soft substrates favor full iPSC reprogramming.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent6609670
dc.identifier.doi10.1038/s41598-019-45352-3
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 18785123
dc.identifier.otherPURE UUID: 95bbca56-9045-4fc5-8cd5-0e1c364f0d44
dc.identifier.otherScopus: 85067838923
dc.identifier.otherPubMed: 31235788
dc.identifier.otherPubMedCentral: PMC6591285
dc.identifier.otherWOS: 000472597100014
dc.identifier.urihttp://hdl.handle.net/10362/105266
dc.identifier.urlhttps://www.scopus.com/pages/publications/85067838923
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F51942%2F2012/PT
dc.subjectGeneral
dc.titleSoft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem/stromal cells (hMSCs) through mechanotransductionen
dc.typejournal article
degois.publication.issue1
degois.publication.titleScientific Reports
degois.publication.volume9
dspace.entity.typePublication
oaire.awardNumberSFRH/BD/51942/2012
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F51942%2F2012/PT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
relation.isProjectOfPublicationcff3f0fe-63e9-4ded-866a-32482e4c2c6e
relation.isProjectOfPublication.latestForDiscoverycff3f0fe-63e9-4ded-866a-32482e4c2c6e

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
s41598_019_45352_3.pdf
Tamanho:
6.3 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

Home collection (FCT)