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Mucins and truncated O-glycans unveil phenotypic discrepancies between serous ovarian cancer cell lines and primary tumours

2018-07-13Artigo científico Acesso aberto
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dc.contributor.authorCoelho, Ricardo
dc.contributor.authorMarcos-Silva, Lara
dc.contributor.authorMendes, Nuno
dc.contributor.authorPereira, Daniela
dc.contributor.authorBrito, Catarina
dc.contributor.authorJacob, Francis
dc.contributor.authorSteentoft, Catharina
dc.contributor.authorMandel, Ulla
dc.contributor.authorClausen, Henrik
dc.contributor.authorDavid, Leonor
dc.contributor.authorRicardo, Sara
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2019-04-26T22:14:06Z
dc.date.available2019-04-26T22:14:06Z
dc.date.issued2018-07-13
dc.description.abstractOptimal research results rely on the selection of cellular models capable of recapitulating the characteristics of primary tumours from which they originate. The expression of mucins (MUC16 and MUC1) and truncated O-glycans (Tn, STn and T) represents a characteristic footprint of serous ovarian carcinomas (SOCs). Therefore, selecting ovarian cancer (OVCA) cell lines that reflect this phenotype is crucial to explore the putative biological role of these biomarkers in the SOC setting. Here, we investigated a panel of OVCA cell lines commonly used as SOC models, and tested whether, when cultured in 2D and 3D conditions, these recapitulate the mucin and O-glycan expression profiles of SOCs. We further explored the role of truncating the O-glycosylation capacity in OVCAR3 cells through knockout of the COSMC chaperone, using in vitro and in vivo assays. We found that the majority of OVCA cell lines of serous origin do not share the mucin and truncated O-glycan footprint of SOCs, although 3D cultures showed a higher resemblance. We also found that genetic truncation of the O-glycosylation capacity of OVCAR3 cells did not enhance oncogenic features either in vitro or in vivo. This study underscores the importance of well-characterized cellular models to study specific features of ovarian cancer.en
dc.description.versionpublished
dc.format.extent4533676
dc.identifier.doi10.3390/ijms19072045
dc.identifier.issn1661-6596
dc.identifier.otherPURE: 12289304
dc.identifier.otherPURE UUID: dbeca308-8b1e-4500-b667-b812fbd08b13
dc.identifier.otherScopus: 85050135212
dc.identifier.otherPubMed: 30011875
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85050135212&partnerID=8YFLogxK
dc.identifier.urlhttps://www.scopus.com/pages/publications/85050135212
dc.language.isoeng
dc.peerreviewedyes
dc.subjectCOSMC
dc.subjectMUC1
dc.subjectMUC16
dc.subjectOvarian cancer cell lines
dc.subjectSerous ovarian carcinomas
dc.subjectTruncated O-glycans
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleMucins and truncated O-glycans unveil phenotypic discrepancies between serous ovarian cancer cell lines and primary tumoursen
dc.typejournal article
degois.publication.issue7
degois.publication.titleInternational Journal of Molecular Sciences
degois.publication.volume19
dspace.entity.typePublication
rcaap.rightsopenAccess

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