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Carbapenemase-producing Enterobacterales strains causing infections in companion animals-Portugal

2024-04Artigo científico Acesso aberto
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dc.contributor.authorda Silva, Joana Moreira
dc.contributor.authorMenezes, Juliana
dc.contributor.authorFernandes, Laura
dc.contributor.authorCosta, Sofia Santos
dc.contributor.authorAmaral, Andreia
dc.contributor.authorPomba, Constança
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblAmerican Society for Microbiology
dc.date.accessioned2024-07-01T22:30:02Z
dc.date.available2024-08-07T00:32:21Z
dc.date.embargoedUntil2024-08-06
dc.date.issued2024-04
dc.descriptionFunding Information: Funding: This research was funded by the Portuguese Foundation for Science and Technology (FCT), under projects UIDB/00276/2020 (CIISA) and LA/P/0059/2020 (AL4AnimalS). J.M.S., J.M., and L.F. were supported by a Fundação para a Ciência e Tecnologia (FCT) PhD fellowship (2020.06540.BD; 2020.07562.BD; UI/BD/153070/2022, respectively). Funding Information: Funding: This research was funded by the Portuguese Foundation for Science and Technology (FCT), under projects UIDB/00276/2020 (CIISA) and LA/P/0059/2020 (AL4AnimalS). Publisher Copyright: Copyright © 2024 Moreira da Silva et al.
dc.description.abstractAn increase in Klebsiella pneumoniae carbapenem-resistant human nosocomial strains is occurring in Europe, namely with the blaOXA-48-like and blaKPClike genes. We determined the prevalence of carbapenemase-producing Enterobacterales clinical strains in companion animals in Portugal and characterized their mobile genetic elements. Susceptibility data of a consecutive collection of 977 Enterobacterales clinical strains from a Portuguese private veterinary diagnostic laboratory were evaluated (January-December 2020). Additional phenotypical and genotypical assays were performed in a subset of 261 strains with a resistant phenotype. Whole-genome sequencing was performed for carbapenemase-producing strains. The frequency of carbapenemase-producing Enterobacterales clinical strains in companion animals in Portugal was 0.51% (n = 5/977). Thus, five strains were characterized: (i) one OXA-181producing K. pneumoniae ST273, (ii) two KPC-3-producing K. pneumoniae ST147; (iii) one KPC-3-producing K. pneumoniae ST392; and (iv) one OXA-48-producing E. coli ST127. The blaKPC-3 gene was located on transposon Tn4401d on IncFIA type plasmid for the K. pneumoniae ST147 strains and on a IncN-type plasmid for the K. pneumoniae ST392 strain, while blaOXA-181 gene was located on an IncX3 plasmid. All de novo assembled plasmids and plasmid-encoded transposons harboring carbapenemase genes were homologous to those previously described in the human healthcare. No plasmid replicons were detected on the OXA-48-producing E. coli ST127. The dissemination of carbapenem resistance is occurring horizontally via plasmid spreading from the human high burden carbapenem resistance setting to the companion animal sector. Furthermore, companion animals may act as reservoirs of carbapenem resistance. Implementation of carbapenemase detection methods in routine clinical veterinary microbiology is urgently needed.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2417247
dc.identifier.doi10.1128/spectrum.03416-23
dc.identifier.issn2165-0497
dc.identifier.otherPURE: 93291560
dc.identifier.otherPURE UUID: 9a0af2f2-2b96-4fb9-899b-a33d58fd465f
dc.identifier.otherScopus: 85189367563
dc.identifier.otherPubMed: 38446073
dc.identifier.otherPubMedCentral: PMC10986603
dc.identifier.otherWOS: WOS:001179410900001
dc.identifier.otherORCID: /0000-0002-4096-2410/work/161969943
dc.identifier.urihttp://hdl.handle.net/10362/169256
dc.identifier.urlhttps://www.scopus.com/pages/publications/85189367563
dc.language.isoeng
dc.peerreviewedyes
dc.subjectdiagnostics
dc.subjectE. coli
dc.subjectKlebsiella pneumoniae
dc.subjectKPC-3
dc.subjectOXA-181
dc.subjectOXA-48
dc.subjectveterinary
dc.subjectPhysiology
dc.subjectEcology
dc.subjectGeneral Immunology and Microbiology
dc.subjectGenetics
dc.subjectMicrobiology (medical)
dc.subjectCell Biology
dc.subjectInfectious Diseases
dc.subjectSDG 3 - Good Health and Well-being
dc.titleCarbapenemase-producing Enterobacterales strains causing infections in companion animals-Portugalen
dc.typejournal article
degois.publication.issue4
degois.publication.titleMicrobiology Spectrum
degois.publication.volume12
dspace.entity.typePublication
rcaap.rightsopenAccess

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