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Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways

2023-03-27Artigo científico Acesso aberto
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dc.contributor.authorEnguita, Francisco J.
dc.contributor.authorPereira, Sofia
dc.contributor.authorLeitão, Ana Lúcia
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionMEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2023-07-12T22:20:23Z
dc.date.available2023-07-12T22:20:23Z
dc.date.issued2023-03-27
dc.descriptionPublisher Copyright: © 2023 by the authors. This research received no external funding
dc.description.abstract(1) Background: Acetaminophen (APAP), an active component of many analgesic and antipyretic drugs, is one of the most concerning trace contaminants in the environment and is considered as an emergent pollutant of marine and aquatic ecosystems. Despite its biodegradability, APAP has become a recalcitrant compound due to the growth of the global population, the ease of availability, and the inefficient wastewater treatment applied. (2) Methods: In this study, we used a transcriptomic approach to obtain functional and metabolic insights about the metabolization of APAP by a phenol-degrading fungal strain, Penicillium chrysogenum var. halophenolicum. (3) Results: We determined that the transcriptomic profile exhibited by the fungal strain during APAP degradation was very dynamic, being characterized by an abundance of dysregulated transcripts which were proportional to the drug metabolization. Using a systems biology approach, we also inferred the protein functional interaction networks that could be related to APAP degradation. We proposed the involvement of intracellular and extracellular enzymes, such as amidases, cytochrome P450, laccases, and extradiol-dioxygenases, among others. (4) Conclusions: Our data suggested that the fungus could metabolize APAP via a complex metabolic pathway, generating nontoxic metabolites, which demonstrated its potential in the bioremediation of this drug.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent20
dc.format.extent4071854
dc.identifier.doi10.3390/jof9040408
dc.identifier.issn2309-608X
dc.identifier.otherPURE: 66105468
dc.identifier.otherPURE UUID: 80090150-b313-4936-b8a7-f31afcb59a3d
dc.identifier.otherScopus: 85153872218
dc.identifier.otherWOS: 000977458600001
dc.identifier.otherPubMed: 37108863
dc.identifier.otherPubMedCentral: PMC10146002
dc.identifier.urihttp://hdl.handle.net/10362/155191
dc.identifier.urlhttps://www.scopus.com/pages/publications/85153872218
dc.language.isoeng
dc.peerreviewedyes
dc.subjectacetaminophen
dc.subjectbiodegradation
dc.subjectfunctional networks
dc.subjectPenicillium chrysogenum
dc.subjecttranscriptomic analysis
dc.subjectEcology, Evolution, Behavior and Systematics
dc.subjectPlant Science
dc.subjectMicrobiology (medical)
dc.subjectSDG 6 - Clean Water and Sanitation
dc.subjectSDG 14 - Life Below Water
dc.titleTranscriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathwaysen
dc.typejournal article
degois.publication.issue4
degois.publication.titleJournal of Fungi
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

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