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An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus

2019-08-22Artigo científico Acesso aberto
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dc.contributor.authorBento-Silva, Andreia
dc.contributor.authorGonçalves, Letice
dc.contributor.authorMecha, Elsa
dc.contributor.authorPereira, Filipe
dc.contributor.authorPatto, Maria Carlota Vaz
dc.contributor.authorBronze, Maria Do Rosário
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.institutionFaculdade de Ciências e Tecnologia (FCT)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2020-11-16T23:58:20Z
dc.date.available2020-11-16T23:58:20Z
dc.date.issued2019-08-22
dc.descriptionThis research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125.
dc.description.abstractβ-N-Oxalyl-l-α,β-diaminopropionic acid (β-ODAP) is a non-protein amino acid present in Lathyrus sativus (grass pea) and other Lathyrus species, in parallel with its nontoxic isomer, α-ODAP. When consuming grass pea for several months as staple food, β-ODAP may cause neurolathyrism, a motor neuron degeneration syndrome. Therefore, the independent quantification of both ODAP isomers instead of only the total amount in grass pea allows the identification of less toxic varieties and the development of tools to support breeding for improving grass pea quality. In this work, a simple and fast HPLC-MS/MS method was developed without sample derivatization, using a hydrophilic interaction chromatography (HILIC) column and an isocratic gradient of eluents for 18 min, which allowed the determination of both α- and β-ODAP. The proposed method was fully validated and applied to the determination of α- and β-ODAP contents in a diverse collection of 107 grass pea accessions representative of the main grass pea-growing geographical regions in the world, with the prompt identification of contrasting accessions. β-ODAP content in the analyzed grass pea samples ranged from 0.45 ± 0.02 to 6.04 ± 0.45 mg g-1. The moderate correlation found between α- and β-ODAP contents (0.65) in this collection reinforces the importance of the independent quantification of both ODAP isomers.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2127933
dc.identifier.doi10.3390/molecules24173043
dc.identifier.otherPURE: 18899587
dc.identifier.otherPURE UUID: 0741b62e-9621-4690-8f6b-84bf5ba53b3b
dc.identifier.otherScopus: 85071493005
dc.identifier.otherPubMed: 31443372
dc.identifier.otherPubMedCentral: PMC6749377
dc.identifier.otherWOS: 000488613700023
dc.identifier.urihttp://hdl.handle.net/10362/107253
dc.identifier.urlhttps://www.scopus.com/pages/publications/85071493005
dc.language.isoeng
dc.peerreviewedyes
dc.subjectGrass pea
dc.subjectHILIC column
dc.subjectHPLC-MS/MS
dc.subjectLathyrus sativus
dc.subjectα-ODAP
dc.subjectβ-ODAP
dc.subjectAnalytical Chemistry
dc.subjectChemistry (miscellaneous)
dc.subjectMolecular Medicine
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.titleAn improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativusen
dc.typejournal article
degois.publication.issue17
degois.publication.titleMolecules
degois.publication.volume24
dspace.entity.typePublication
rcaap.rightsopenAccess

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