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Olive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applications

2021-10-02Artigo científico Acesso aberto
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dc.contributor.authorKatsinas, Nikolaos
dc.contributor.authorEnríquez-De-salamanca, Amalia
dc.contributor.authorSilva, Andreia Bento da
dc.contributor.authorBronze, Maria Rosário
dc.contributor.authorRodríguez-Rojo, Soraya
dc.contributor.institutionDCT - Departamento de Ciências da Terra
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-01-22T03:41:33Z
dc.date.available2022-01-22T03:41:33Z
dc.date.issued2021-10-02
dc.descriptionH2020-MSCA-ITN-2017 01 0145 FEDER 402 022125
dc.description.abstractNowadays, increasing interest in olive pomace (OP) valorization aims to improve olive’s industry sustainability. Interestingly, several studies propose a high-value application for OP extracts containing its main phenolic compounds, hydroxytyrosol and oleuropein, as therapy for ocular surface diseases. In this work, the stability and accessibility of OP total phenolic and flavonoid content, main representative compounds, and antioxidant activity were assessed under different pretreatment conditions. Among them, lyophilization and supercritical CO2 extraction were found to increase significantly most responses measured in the produced extracts. Two selected extracts (CONV and OPT3) were obtained by different techniques (conventional and pressurized liquid ex-traction); Their aqueous solutions were characterized by HPLC-DAD-MS/MS. Additionally, their safety and stability were evaluated according to EMA requirements towards their approval as ophthalmic products: their genotoxic effect on ocular surface cells and their 6-months storage stability at 4 different temperature/moisture conditions (CPMP/ICH/2736/99), together with pure hydroxy-tyrosol and oleuropein solutions. The concentration of hydroxytyrosol and oleuropein in pure or extract solutions was tracked, and possible degradation products were putatively identified by HPLC-DAD-MS/MS. Hydroxytyrosol and oleuropein had different stability as standard or extract solutions, with oleuropein also showing different degradation profile. All compounds/extracts were safe for ophthalmic use at the concentrations tested.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2413588
dc.identifier.doi10.3390/molecules26196002
dc.identifier.issn1420-3049
dc.identifier.otherPURE: 35917588
dc.identifier.otherPURE UUID: 0e068b75-e62b-4e62-9f80-c7d37ef93823
dc.identifier.otherScopus: 85116550513
dc.identifier.otherPubMed: 34641545
dc.identifier.otherPubMedCentral: PMC8512844
dc.identifier.otherWOS: 000717538200001
dc.identifier.urihttp://hdl.handle.net/10362/131330
dc.identifier.urlhttps://www.scopus.com/pages/publications/85116550513
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/765608/EU
dc.relationIntegrated Training in Dry Eye Disease Drug Development (IT-DED3)
dc.subjectGenotoxicity assay
dc.subjectHydroxytyrosol
dc.subjectOleuropein
dc.subjectOlive pomace
dc.subjectPhenolic extracts
dc.subjectStorage stability
dc.subjectAnalytical Chemistry
dc.subjectChemistry (miscellaneous)
dc.subjectMolecular Medicine
dc.subjectPharmaceutical Science
dc.subjectDrug Discovery
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.titleOlive pomace phenolic compounds stability and safety evaluation: From raw material to future ophthalmic applicationsen
dc.typejournal article
degois.publication.issue19
degois.publication.titleMolecules
degois.publication.volume26
dspace.entity.typePublication
oaire.awardNumber765608
oaire.awardTitleIntegrated Training in Dry Eye Disease Drug Development (IT-DED3)
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/765608/EU
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccess
relation.isProjectOfPublicationabec630a-c5b8-4dc9-b7d5-f876a46092a9
relation.isProjectOfPublication.latestForDiscoveryabec630a-c5b8-4dc9-b7d5-f876a46092a9

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