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Next-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)

2020-05Artigo científico Acesso aberto
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dc.contributor.authorSabença, Carolina
dc.contributor.authorde Sousa, Telma
dc.contributor.authorOliveira, Soraia
dc.contributor.authorViala, Didier
dc.contributor.authorThéron, Laetitia
dc.contributor.authorChambon, Christophe
dc.contributor.authorHébraud, Michel
dc.contributor.authorBeyrouthy, Racha
dc.contributor.authorBonnet, Richard
dc.contributor.authorCaniça, Manuela
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-06-25T22:18:36Z
dc.date.available2021-06-25T22:18:36Z
dc.date.issued2020-05
dc.descriptionAção n.º: TC-14 / 2017 UID/QUI/50006/2020 UIDB/00211/2020 UMR1071 USC-2018
dc.description.abstractVancomycin-resistant enterococci (VRE), due to their intrinsic resistance to various commonly used antibiotics and their malleable genome, make the treatment of infections caused by these bacteria less effective. The aims of this work were to characterize isolates of Enterococcus spp. that originated from processed meat, through phenotypic and genotypic techniques, as well as to detect putative antibiotic resistance biomarkers. The 19 VRE identified had high resistance to teicoplanin (89%), tetracycline (94%), and erythromycin (84%) and a low resistance to kanamycin (11%), gentamicin (11%), and streptomycin (5%). Based on a Next-Generation Sequencing NGS technique, most isolates were vanA-positive. The most prevalent resistance genes detected were erm(B) and aac(6')-Ii, conferring resistance to the classes of macrolides and aminoglycosides, respectively. MALDI-TOF mass spectrometry (MS) analysis detected an exclusive peak of the Enterococcus genus at m/z (mass-to-charge-ratio) 4428 ± 3, and a peak at m/z 6048 ± 1 allowed us to distinguish Enterococcus faecium from the other species. Several statistically significant protein masses associated with resistance were detected, such as peaks at m/z 6358.27 and m/z 13237.3 in ciprofloxacin resistance isolates. These results reinforce the relevance of the combined and complementary NGS and MALDI-TOF MS techniques for bacterial characterization.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2205407
dc.identifier.doi10.3390/biology9050089
dc.identifier.otherPURE: 32184625
dc.identifier.otherPURE UUID: b5f4f637-58cc-4338-98e7-a927c6aeb53c
dc.identifier.otherScopus: 85084118807
dc.identifier.otherPubMed: 32349310
dc.identifier.otherPubMedCentral: PMC7284646
dc.identifier.otherWOS: 000541023400010
dc.identifier.urihttp://hdl.handle.net/10362/120027
dc.identifier.urlhttps://www.scopus.com/pages/publications/85084118807
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAntibiotic resistance
dc.subjectEnterococcus spp
dc.subjectMALDI-TOF MS
dc.subjectNext-generation sequencing
dc.subjectProcessed meat
dc.subjectGeneral Biochemistry,Genetics and Molecular Biology
dc.subjectGeneral Immunology and Microbiology
dc.subjectGeneral Agricultural and Biological Sciences
dc.titleNext-generation sequencing and maldi mass spectrometry in the study of multiresistant processed meat vancomycin-resistant enterococci (VRE)en
dc.typejournal article
degois.publication.issue5
degois.publication.titleBiology
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

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