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Isothermal Amplification of Nucleic Acids

2021Recensão Acesso aberto
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dc.contributor.authorOliveira, Beatriz B.
dc.contributor.authorVeigas, Bruno
dc.contributor.authorBaptista, Pedro Viana
dc.contributor.institutionDCV - Departamento de Ciências da Vida
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblFrontiers Media
dc.date.accessioned2024-10-31T23:07:08Z
dc.date.available2024-10-31T23:07:08Z
dc.date.issued2021
dc.descriptionPublisher Copyright: Copyright © 2021 Oliveira, Veigas and Baptista.
dc.description.abstractNucleic acid amplification technologies (NAATs) have become fundamental tools in molecular diagnostics, due to their ability to detect small amounts of target molecules. Since its development, Polymerase Chain Reaction (PCR) has been the most exploited method, being stablished as the “gold standard” technique for DNA amplification. However, the requirement for different working temperatures leads to the need of a thermocycler machine or complex thermal apparatus, which have been preventing its application in novel integrated devices for single workflow and high throughput analysis. Conversely, isothermal amplification methods have been gaining attention, especially for point-of-care diagnosis and applications. These non-PCR based methods have been developed by mimicking the in vivo amplification mechanisms, while performing the amplification with high sensitivity, selectivity and allowing for high-throughput analysis. These favorable capabilities have pushed forward the implementation and commercialization of several platforms that exploit isothermal amplification methods, mostly against virus, bacteria and other pathogens in water, food, environmental and clinical samples. Nevertheless, the future of isothermal amplification methods is still dependent on achieving technical maturity and broader commercialization of enzymes and reagents.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent22
dc.format.extent1983744
dc.identifier.doi10.3389/fsens.2021.752600
dc.identifier.issn2673-5067
dc.identifier.otherPURE: 99281355
dc.identifier.otherPURE UUID: e364689e-d944-40ad-a11a-2beb98d5df1b
dc.identifier.otherScopus: 85196214988
dc.identifier.urihttp://hdl.handle.net/10362/174418
dc.identifier.urlhttps://www.scopus.com/pages/publications/85196214988
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
dc.relationApplied Molecular Biosciences Unit
dc.relationinfo:eu-repo/grantAgreement/FCT//2020.07660.BD/PT
dc.subjectisothermal amplification
dc.subjectNAAT
dc.subjectnon-pcr amplification
dc.subjectnucleic acids amplification
dc.subjectpoint—of—care
dc.subjectAnalytical Chemistry
dc.subjectBiomedical Engineering
dc.subjectElectrical and Electronic Engineering
dc.subjectMaterials Science (miscellaneous)
dc.titleIsothermal Amplification of Nucleic Acidsen
dc.title.subtitleThe Race for the Next “Gold Standard”en
dc.typereview
degois.publication.titleFrontiers in Sensors
degois.publication.volume2
dspace.entity.typePublication
oaire.awardNumberUIDB/04378/2020
oaire.awardNumberLA/P/0140/2020
oaire.awardNumber2020.07660.BD
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)/LA%2FP%2F0140%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07660.BD/PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Atribuição do Estatuto e Financiamento de Laboratórios Associados (LA)
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
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relation.isProjectOfPublication.latestForDiscovery18f3a9d5-ef8b-445d-9b08-45c07f1f8b48

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