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Probing CHO cells metabolism using metabolomics and fluxomics tools
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Resumo(s)
Chinese hamster ovary (CHO) cells are preferred mammalian hosts for industrial
production of therapeutic glycoproteins such as monoclonal antibodies (mAbs), used to
treat cancer and immunological disorders. In these cells, the glutamine synthetase (GS)
expression system has been widely used for efficient selection of high-yielding clones.
However, fundamental knowledge on the metabolic behavior of GS-CHO cells in culture
and its impact on product yields have not been yet systematized. The overall objective
of this thesis was to comprehensively analyse the metabolome and fluxome of CHO
cells exploring how the metabolism is affected by clonal variability and culture
conditions. This information is then used to generate hypotheses on how metabolic
wiring impacts mAb production.(...)
Descrição
Palavras-chave
Metabolomics Fluxomics