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Molecular Epidemiology of Rotavirus Strains in Symptomatic and Asymptomatic Children in Manhiça District, Southern Mozambique 2008–2019

2022-01Artigo científico Acesso aberto
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dc.contributor.authorManjate, Filomena
dc.contributor.authorJoão, Eva D.
dc.contributor.authorChirinda, Percina
dc.contributor.authorGarrine, Marcelino
dc.contributor.authorVubil, Delfino
dc.contributor.authorNobela, Nélio
dc.contributor.authorKotloff, Karen
dc.contributor.authorNataro, James P.
dc.contributor.authorNhampossa, Tacilta
dc.contributor.authorAcácio, Sozinho
dc.contributor.authorTate, Jacqueline E.
dc.contributor.authorParashar, Umesh
dc.contributor.authorMwenda, Jason M.
dc.contributor.authorAlonso, Pedro L.
dc.contributor.authorNyaga, Martin
dc.contributor.authorCunha, Celso
dc.contributor.authorMandomando, Inácio
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionTB, HIV and opportunistic diseases and pathogens (THOP)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2022-02-08T23:22:56Z
dc.date.available2022-02-08T23:22:56Z
dc.date.issued2022-01
dc.description..870-15 SC; the United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investiga??o (FNI), Mo?ambique, grant number 245-INV, within the context of diarrhoeal disease surveillance platform implementation. F.M PhD is supported by Calouste Gulbenkian Foundation, grant number 234066. The authors convey many thanks to all the caregivers who consented to their children?s participation in both studies (GEMS and the diarrhoeal disease platform). They would also like to thank all the professionals in the hospitals and those on field recruitment for their full dedication and effort in children enrolment and collection of data and samples whenever possible. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractGroup A rotaviruses remain the leading cause of diarrhoea in children aged <5 years. Mozambique introduced rotavirus vaccine (Rotarix® ) in September 2015. We report rotavirus geno-types circulating among symptomatic and asymptomatic children in Manhiça District, Mozambique, pre-and post-vaccine introduction. Stool was collected from enrolled children and screened for ro-tavirus by enzyme-immuno-sorbent assay. Positive specimens were genotyped for VP7 (G genotypes) and VP4 (P genotypes) by the conventional reverse transcriptase polymerase chain reaction. The combination G12P[8] was more frequently observed in pre-vaccine than in post-vaccine introduction, in moderate to severe diarrhoea (34%, 61/177 vs. 0, p < 0.0001) and controls (23%, 26/113 vs. 0, p = 0.0013) and mixed genotypes (36%, 24/67 vs. 7% 4/58, p = 0.0003) in less severe diarrhoea. We observed changes in post-vaccine compared to pre-vaccine introduction, where G3P[4] and G3P[8] were prevalent in moderate to severe diarrhoea (10%, 5/49 vs. 0, p = 0.0002; and 14%, 7/49 vs. 1%, 1/177, p < 0.0001; respectively), and in less severe diarrhoea (21%, 12/58 vs. 0, p = 0.003; and 24%, 14/58 vs. 0, p < 0.0001; respectively). Our surveillance demonstrated the circulation of similar genotypes contemporaneously among cases and controls, as well as switching from pre-to post-vaccine introduction. Continuous surveillance is needed to evaluate the dynamics of the changes in genotypes following vaccine introduction.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent469496
dc.identifier.doi10.3390/v14010134
dc.identifier.issn1999-4915
dc.identifier.otherPURE: 36376352
dc.identifier.otherPURE UUID: 9ec37ee1-e79c-449c-a3a6-55c54737339e
dc.identifier.otherScopus: 85123016430
dc.identifier.otherORCID: /0000-0003-4745-3819/work/107026490
dc.identifier.urihttp://hdl.handle.net/10362/132538
dc.identifier.urlhttps://www.scopus.com/pages/publications/85123016430
dc.language.isoeng
dc.peerreviewedyes
dc.relationFunding Information: Funding: The GEMS study was funded by the Bill & Melinda Gates Foundation (OPP1033572) through the Center for Vaccine Development, USA. The surveillance of rotavirus and other en-teropathogens in children less than 5 years of age in Manhiça was supported by GAVI through the Centers for Disease Control and Prevention (CDC), Atlanta, and the World Health Organization, Regional Office for Africa (WHO/AFRO), grant number MOA
dc.relation..870-15 SC; the United States Agency for International Development (USAID), grant number AID-656-F-16-00002 and Fundo Nacional de Investigação (FNI), Moçambique, grant number 245-INV, within the context of diarrhoeal disease surveillance platform implementation. F.M PhD is supported by Calouste Gulbenkian Foundation, grant number 234066. Funding Information: The GEMS study was funded by the Bill & Melinda Gates Foundation (OPP1033572) through the Center for Vaccine Development, USA. The surveillance of rotavirus and other en-teropathogens in children less than 5 years of age in Manhi?a was supported by GAVI through the Centers for Disease Control and Prevention (CDC), Atlanta, and the World Health Organization, Regional Office for Africa (WHO/AFRO), grant number MOA
dc.subjectCase-control study
dc.subjectGenotypes
dc.subjectMozambique
dc.subjectRotavirus A
dc.subjectInfectious Diseases
dc.subjectVirology
dc.subjectSDG 3 - Good Health and Well-being
dc.subjectSDG 10 - Reduced Inequalities
dc.subjectSDG 6 - Clean Water and Sanitation
dc.subjectSDG 14 - Life Below Water
dc.titleMolecular Epidemiology of Rotavirus Strains in Symptomatic and Asymptomatic Children in Manhiça District, Southern Mozambique 2008–2019en
dc.typejournal article
degois.publication.issue1
degois.publication.titleViruses
degois.publication.volume14
dspace.entity.typePublication
rcaap.rightsopenAccess

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