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The impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial cultures

2022-01-25Artigo científico Acesso aberto
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dc.contributor.authorCruz, Rafaela A. P.
dc.contributor.authorOehmen, Adrian
dc.contributor.authorReis, Maria A. M.
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionUCIBIO - Applied Molecular Biosciences Unit
dc.contributor.pblElsevier
dc.date.accessioned2022-03-11T23:22:34Z
dc.date.available2022-03-11T23:22:34Z
dc.date.issued2022-01-25
dc.descriptionUIDP/04378/2020 UIDB/04378/2020 LA/P/0140/2020 SFRH/BD/110673/2015
dc.description.abstractThe production of polyhydroxyalkanoates (PHA) by mixed microbial cultures (MMC) has been studied as an alternative to pure cultures in order to reduce the price of PHA through use of open systems and low-cost substrates, such as agro-industrial sub-products. However, the widespread applicability of this process depends on the optimization of operational factors impacting PHA productivity. This study addresses the impact of biomass withdrawal strategy on the performance of MMC selection reactors and consequently on biomass productivity and global PHA productivity. Two selection reactors were operated in parallel under similar conditions, except for the timing of biomass withdrawal, at the end of the famine phase (Reactor 1, R1) versus at the end of the feast phase (Reactor 2, R2) at an organic loading rate of 100 Cmmol.L−1.d−1 and solids retention time of 4 days. The biomass selected in both conditions had similar PHA storing capacity as shown by the similar yields of PHA per substrate obtained in the accumulation assays; however, R1 reached a higher biomass productivity (about 4-fold higher than R2). This study demonstrated that removing the excess biomass at the end of the famine phase resulted in a much higher global PHA productivity and that the key parameter affecting the global PHA productivity of the 2-stage system was the volumetric biomass productivity. Results obtained provide important insight into how MMC systems can be best operated to maximize PHA productivity.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent8
dc.format.extent1556964
dc.identifier.doi10.1016/j.nbt.2021.08.004
dc.identifier.issn1871-6784
dc.identifier.otherPURE: 36690863
dc.identifier.otherPURE UUID: a55a409b-60b2-4839-940e-1e4010f772ec
dc.identifier.otherScopus: 85113686928
dc.identifier.otherPubMed: 34450342
dc.identifier.otherWOS: 000731397200002
dc.identifier.urihttp://hdl.handle.net/10362/134345
dc.identifier.urlhttps://www.scopus.com/pages/publications/85113686928
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBiomass withdrawal
dc.subjectBioplastics
dc.subjectMixed microbial cultures (MMC)
dc.subjectPolyhydroxyalkanoates (PHA)
dc.subjectProcess optimization
dc.subjectSequencing batch reactors (SBR)
dc.subjectBiotechnology
dc.subjectBioengineering
dc.subjectMolecular Biology
dc.titleThe impact of biomass withdrawal strategy on the biomass selection and polyhydroxyalkanoates accumulation of mixed microbial culturesen
dc.typejournal article
degois.publication.firstPage8
degois.publication.lastPage15
degois.publication.titleNew Biotechnology
degois.publication.volume66
dspace.entity.typePublication
rcaap.rightsopenAccess

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