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NMR-metabolomics shows that bola is an important modulator of Salmonella typhimurium metabolic processes under virulence conditions

2019-11-01Artigo cientĆ­fico Acesso aberto
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dc.contributor.authorGraƧaā€lopes, Gil
dc.contributor.authorGraƧa, GonƧalo
dc.contributor.authorBarahona, Susana
dc.contributor.authorMoreira, Ricardo N.
dc.contributor.authorArraiano, CecĆ­lia M.
dc.contributor.authorGonƧalves, Luƭs G.
dc.contributor.institutionInstituto de Tecnologia QuĆ­mica e BiolĆ³gica AntĆ³nio Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2020-02-04T23:41:24Z
dc.date.available2020-02-04T23:41:24Z
dc.date.issued2019-11-01
dc.description.abstractBolA is a ubiquitous global transcription factor. Despite its clear role in the induction of important stressā€resistant physiological changes and its recent implication in the virulence of Salmonella, further research is required to shed light on the pathways modulated by BolA. In this study, we resorted to untargeted1Hā€NMR metabolomics to understand the impact of BolA on the metabolic profile of Salmonella Typhimurium, under virulence conditions. Three strains of S. Typhimurium SL1344 were studied: An SL1344 strain transformed with an empty plasmid (control), a bolA knockout mutant (Ī”bolA), and a strain overexpressing bolA (bolA+). These strains were grown in a minimal virulenceā€inducing medium and cells were collected at the end of the exponential and stationary phases. The extracts were analyzed by NMR, and multivariate and univariate statistical analysis were performed to identify significant alterations. Principal component analysis (PCA) and partial least squares discriminant analysis (PLSā€DA) of1Hā€NMR data allowed the discrimination between the metabolic profiles of these strains, revealing increased levels of acetate, valine, alanine, NAD+, succinate, coenzyme A, glutathione, and putrescine in bolA+. These results indicate that BolA regulates pathways related to stress resistance and virulence, being an important modulator of the metabolic processes needed for S. Typhimurium infection.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2397764
dc.identifier.doi10.3390/metabo9110243
dc.identifier.issn2218-1989
dc.identifier.otherPURE: 15805673
dc.identifier.otherPURE UUID: e58d4de5-db3e-4b22-a5e7-65c161c23b7b
dc.identifier.otherScopus: 85074230813
dc.identifier.otherORCID: /0000-0001-5683-3552/work/68656617
dc.identifier.urihttp://hdl.handle.net/10362/92209
dc.identifier.urlhttps://www.scopus.com/pages/publications/85074230813
dc.identifier.urlhttps://www.mdpi.com/2218-1989/9/11/243
dc.language.isoeng
dc.peerreviewedyes
dc.subjectBolA
dc.subjectMetabolomics
dc.subjectNMR
dc.subjectSalmonella Typhimurium
dc.subjectVirulence
dc.subjectEndocrinology, Diabetes and Metabolism
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleNMR-metabolomics shows that bola is an important modulator of Salmonella typhimurium metabolic processes under virulence conditionsen
dc.typejournal article
degois.publication.issue11
degois.publication.titleMetabolites
degois.publication.volume9
dspace.entity.typePublication
rcaap.rightsopenAccess

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