Logo do repositório
 
Publicação

Selective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensor

2020-09-22Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorMoro, Artur J.
dc.contributor.authorSantos, Miguel
dc.contributor.authorOutis, Mani
dc.contributor.authorMateus, Pedro
dc.contributor.authorPereira, Pedro Matos
dc.contributor.institutionLAQV@REQUIMTE
dc.contributor.institutionDQ - Departamento de Química
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-05-03T22:53:20Z
dc.date.available2021-05-03T22:53:20Z
dc.date.issued2020-09-22
dc.descriptionUIDB/50006/2020 SFRH/BD/120985/2016 Project LISBOA-01-0145-FEDER-007660 PPBI-POCI-01-0145-FEDER-022122 DL 57-021/DL/2018 ROTEIRO/0031/2013-PINFRA/22161/2016
dc.description.abstractA new fluorescent chemosensor for copper (II) and subsequent anion sensing was designed and fully characterized. The sensor consisted of a 1,8-naphthalimide core, bearing two terminal dipicolylamine (DPA) receptor units for binding metal cations, and an ethoxyethanol moiety for enhanced water solubility. The DPA units are connected to position 4 of the fluorophore via a triazine-ethylenediamine spacer. Fluorescence titration studies of the chemosensor revealed a high selectivity for Cu2+ over other divalent ions, the emissions were strongly quenched upon binding, and a stability constant of 5.52 log units was obtained. Given the distance from DPA chelating units and the fluorophore, quenching from the Cu2+ complexation suggests an electron transfer or an electronic energy transfer mechanism. Furthermore, the Cu2+-sensor complex proved to be capable of sensing anionic phosphate derivatives through the displacement of the Cu2+ cation, which translated into a full recovery of the luminescence from the naphthalimide. Super-resolution fluorescence microscopy studies performed in HeLa cells showed there was a high intracellular uptake of the chemosensor. Incubation in Cu2+ spiked media revealed a strong fluorescent signal from mitochondria and cell membranes, which is consistent with a high concentration of ATP at these intracellular sites.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent3234543
dc.identifier.doi10.3390/bios10090129
dc.identifier.issn2079-6374
dc.identifier.otherPURE: 20124686
dc.identifier.otherPURE UUID: 8d57e303-2a8a-4410-a803-b5bceed51d55
dc.identifier.otherScopus: 85091619808
dc.identifier.otherPubMed: 32971802
dc.identifier.otherPubMedCentral: PMC7558417
dc.identifier.otherWOS: 000578179600001
dc.identifier.otherORCID: /0000-0001-8973-1595/work/93186239
dc.identifier.urihttp://hdl.handle.net/10362/116889
dc.identifier.urlhttps://www.scopus.com/pages/publications/85091619808
dc.language.isoeng
dc.peerreviewedyes
dc.subjectcopper (II)
dc.subjectfluorescent chemosensor
dc.subjectnaphthalimide
dc.subjectphosphate derivatives
dc.subjectsuper-resolution fluorescence microscopy
dc.subjectClinical Biochemistry
dc.titleSelective Coordination of Cu2+ and Subsequent Anion Detection Based on a Naphthalimide-Triazine-(DPA)2 Chemosensoren
dc.typejournal article
degois.publication.issue9
degois.publication.titleBiosensors
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
biosensors_10_00129.pdf
Tamanho:
3.08 MB
Formato:
Adobe Portable Document Format
Ver/Abrir

Coleções

FCT: DQ - Artigos em revista internacional com arbitragem científica