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The Hypervariable Tpr Multigene Family of Theileria Parasites, Defined by a Conserved, Membrane-Associated, C-Terminal Domain, Includes Several Copies with Defined Orthology Between Species

2023-12Artigo científico Acesso aberto
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dc.contributor.authorPalmateer, Nicholas C.
dc.contributor.authorMunro, James B.
dc.contributor.authorNagaraj, Sushma
dc.contributor.authorCrabtree, Jonathan
dc.contributor.authorPelle, Roger
dc.contributor.authorTallon, Luke
dc.contributor.authorNene, Vish
dc.contributor.authorBishop, Richard
dc.contributor.authorSilva, Joana C.
dc.contributor.institutionInstituto de Higiene e Medicina Tropical (IHMT)
dc.contributor.institutionVector borne diseases and pathogens (VBD)
dc.contributor.institutionGlobal Health and Tropical Medicine (GHTM)
dc.contributor.pblSpringer Science Business Media
dc.date.accessioned2024-03-14T23:58:52Z
dc.date.available2024-03-14T23:58:52Z
dc.date.issued2023-12
dc.descriptionFunding Information: Funding for the work was provided by the Bill and Melinda Gates Foundation (US) (OPP1078791 to VN), the Agricultural Research Service (59–5348–4-001, with cooperative agreement to JCS), and the National Institute of Allergy and Infectious Diseases (R01AI141900 to JCS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding Information: All sequencing data were generated by Maryland Genomics, Institute for Genome Sciences, University of Maryland School of Medicine. Publisher Copyright: © 2023, The Author(s).
dc.description.abstractMultigene families often play an important role in host-parasite interactions. One of the largest multigene families in Theileria parva, the causative agent of East Coast fever, is the T. parva repeat (Tpr) gene family. The function of the putative Tpr proteins remains unknown. The initial publication of the T. parva reference genome identified 39 Tpr family open reading frames (ORFs) sharing a conserved C-terminal domain. Twenty-eight of these are clustered in a central region of chromosome 3, termed the “Tpr locus”, while others are dispersed throughout all four nuclear chromosomes. The Tpr locus contains three of the four assembly gaps remaining in the genome, suggesting the presence of additional, as yet uncharacterized, Tpr gene copies. Here, we describe the use of long-read sequencing to attempt to close the gaps in the reference assembly of T. parva (located among multigene families clusters), characterize the full complement of Tpr family ORFs in the T. parva reference genome, and evaluate their evolutionary relationship with Tpr homologs in other Theileria species. We identify three new Tpr family genes in the T. parva reference genome and show that sequence similarity among paralogs in the Tpr locus is significantly higher than between genes outside the Tpr locus. We also identify sequences homologous to the conserved C-terminal domain in five additional Theileria species. Using these sequences, we show that the evolution of this gene family involves conservation of a few orthologs across species, combined with gene gains/losses, and species-specific expansions.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent3527212
dc.identifier.doi10.1007/s00239-023-10142-z
dc.identifier.issn0022-2844
dc.identifier.otherPURE: 82961918
dc.identifier.otherPURE UUID: 2f90c41f-48e1-401d-9b1f-ba46b11e38ef
dc.identifier.otherScopus: 85177860516
dc.identifier.otherWOS: 001108566300001
dc.identifier.otherPubMedCentral: PMC10730637
dc.identifier.otherPubMed: 38017120
dc.identifier.urihttp://hdl.handle.net/10362/164970
dc.identifier.urlhttps://www.scopus.com/pages/publications/85177860516
dc.language.isoeng
dc.peerreviewedyes
dc.subjectAssembly gaps
dc.subjectLineage-specific expansion
dc.subjectMultigene family
dc.subjectTheileria parva
dc.subjectEcology, Evolution, Behavior and Systematics
dc.subjectMolecular Biology
dc.subjectGenetics
dc.subjectParasitology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleThe Hypervariable Tpr Multigene Family of Theileria Parasites, Defined by a Conserved, Membrane-Associated, C-Terminal Domain, Includes Several Copies with Defined Orthology Between Speciesen
dc.typejournal article
degois.publication.firstPage897
degois.publication.issue6
degois.publication.lastPage911
degois.publication.titleJournal Of Molecular Evolution
degois.publication.volume91
dspace.entity.typePublication
rcaap.rightsopenAccess

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