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Serr spectroelectrochemistry as a guide for rational design of dyp-based bioelectronics devices

2021-08-01Artigo científico Acesso aberto
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dc.contributor.authorZuccarello, Lidia
dc.contributor.authorBarbosa, Catarina
dc.contributor.authorGaldino, Edilson
dc.contributor.authorLončar, Nikola
dc.contributor.authorSilveira, Célia M.
dc.contributor.authorFraaije, Marco W.
dc.contributor.authorTodorovic, Smilja
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblMDPI - Multidisciplinary Digital Publishing Institute
dc.date.accessioned2021-12-06T23:43:25Z
dc.date.available2021-12-06T23:43:25Z
dc.date.issued2021-08-01
dc.descriptionFunding Information: Acknowledgments: We acknowledge the support from Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE 2020-Programa Operacional Competitividade e Internacionalização (POCI), from FCT—Fundação para a Ciência e a Tecnologia (PTDC/BIA-BFS/31026/2017 and 2020.05017.BD) and from the European Union’s Horizon 2020 Research and Innovation Program, through TIMB3 and B-LigZymes projects (grant agreements numbers are 810856 and 824017, respectively). Molecular graphics and analyses were performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. Funding Information: This research was funded by FCT?Funda??o para a Ci?ncia e a Tecnologia (grant number: PTDC/BIA-BFS/31026/2017). The APC was funded by the TIMB3 project, European Union?s Horizon 2020 Research and Innovation Program (grant agreement No.: 810856). Funding Information: Funding: This research was funded by FCT—Fundação para a Ciência e a Tecnologia (grant number: PTDC/BIA-BFS/31026/2017). The APC was funded by the TIMB3 project, European Union’s Horizon 2020 Research and Innovation Program (grant agreement No.: 810856). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractImmobilised dye-decolorizing peroxidases (DyPs) are promising biocatalysts for the development of biotechnological devices such as biosensors for the detection of H2O2 . To this end, these enzymes have to preserve native, solution properties upon immobilisation on the electrode surface. In this work, DyPs from Cellulomonas bogoriensis (CboDyP), Streptomyces coelicolor (ScoDyP) and Thermobifida fusca (TfuDyP) are immobilised on biocompatible silver electrodes functionalized with alkanethiols. Their structural, redox and catalytic properties upon immobilisation are evaluated by surface-enhanced resonance Raman (SERR) spectroelectrochemistry and cyclic voltammetry. Among the studied electrode/DyP constructs, only CboDyP shows preserved native structure upon attachment to the electrode. However, a comparison of the redox potentials of the enzyme in solution and immobilised states reveals a large discrepancy, and the enzyme shows no electrocatalytic activity in the presence of H2O2 . While some immobilised DyPs outperform existing peroxidase-based biosensors, others fail to fulfil the essential requirements that guarantee their applicability in the im-mobilised state. The capacity of SERR spectroelectrochemistry for fast screening of the performance of immobilised heme enzymes places it in the front-line of experimental approaches that can advance the search for promising DyP candidates.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent2246082
dc.identifier.doi10.3390/ijms22157998
dc.identifier.issn1661-6596
dc.identifier.otherPURE: 35228576
dc.identifier.otherPURE UUID: 659f261e-d030-42d1-a6ee-209282fbd5fe
dc.identifier.otherScopus: 85111095678
dc.identifier.otherPubMed: 34360763
dc.identifier.urihttp://hdl.handle.net/10362/128805
dc.identifier.urlhttps://www.scopus.com/pages/publications/85111095678
dc.language.isoeng
dc.peerreviewedyes
dc.subject3 generation biosensors
dc.subjectDyP
dc.subjectImmobilised enzymes
dc.subjectSERR spectroelectrochemistry
dc.subjectCatalysis
dc.subjectMolecular Biology
dc.subjectSpectroscopy
dc.subjectComputer Science Applications
dc.subjectPhysical and Theoretical Chemistry
dc.subjectOrganic Chemistry
dc.subjectInorganic Chemistry
dc.titleSerr spectroelectrochemistry as a guide for rational design of dyp-based bioelectronics devicesen
dc.typejournal article
degois.publication.issue15
degois.publication.titleInternational Journal of Molecular Sciences
degois.publication.volume22
dspace.entity.typePublication
rcaap.rightsopenAccess

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