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Rotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthases

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dc.contributor.authorValdivieso González, David
dc.contributor.authorMakowski, Marcin
dc.contributor.authorLillo, M. Pilar
dc.contributor.authorCao-García, Francisco J.
dc.contributor.authorMelo, Manuel N.
dc.contributor.authorAlmendro-Vedia, Víctor G.
dc.contributor.authorLópez-Montero, Iván
dc.contributor.institutionInstituto de Tecnologia Química e Biológica António Xavier (ITQB)
dc.contributor.pblWiley-VCH Verlag
dc.date.accessioned2024-03-31T23:34:07Z
dc.date.available2024-03-31T23:34:07Z
dc.date.issued2023
dc.descriptionFunding Information: D.V.G. and M.M. contributed equally to this work and are co‐first authors. I.L.‐M. and M.P.L. acknowledge financial support from the Spanish Ministry of Science, Innovation and Universities through the grant PGC2018‐097903‐B‐I00. This work was also supported by the TECNOLOGÍAS 2018 program funded by the Regional Government of Madrid (Grant S2018/BAA‐4403 SINOXPHOS‐CM) and PID2021‐125024NB‐C22 (To I.L.‐M.). DVG thanks the Regional Government of Madrid for an assistant researcher contract (CT103/19/PEJ‐2019‐AI/IND‐13687). M.M. acknowledges Fundação para a Ciência e a Tecnologia's (FCT) fellowship SPRH/BD/128290/2017. M.N.M. acknowledges financial support from FCT for fellowship CEECIND/04124/2017 and project MOSTMICRO‐ITQB, with references UIDB/04612/2020 and UIDP/04612/2020. F.J.C.‐G. was supported by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund through grant RTI2018‐095802‐B‐I00. V.G.A.‐V. thanks the Regional Government of Madrid, Universidad Complutense de Madrid and Fundación Complutense for computational resources (by Grant PR27/21 030). I.L.‐M. thanks Dr. Frédéric Pincet and Dr. Paolo Natale for fruitful discussions. Publisher Copyright: © 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.
dc.description.abstractATP synthases are proteins that catalyse the formation of ATP through the rotatory movement of their membrane-spanning subunit. In mitochondria, ATP synthases are found to arrange as dimers at the high-curved edges of cristae. Here, a direct link is explored between the rotatory movement of ATP synthases and their preference for curved membranes. An active curvature sorting of ATP synthases in lipid nanotubes pulled from giant vesicles is found. Coarse-grained simulations confirm the curvature-seeking behaviour of rotating ATP synthases, promoting reversible and frequent protein-protein contacts. The formation of transient protein dimers relies on the membrane-mediated attractive interaction of the order of 1.5 kBT produced by a hydrophobic mismatch upon protein rotation. Transient dimers are sustained by a conic-like arrangement characterized by a wedge angle of θ ≈ 50°, producing a dynamic coupling between protein shape and membrane curvature. The results suggest a new role of the rotational movement of ATP synthases for their dynamic self-assembly in biological membranes.en
dc.description.versionpublishersversion
dc.description.versioninpress
dc.format.extent2364053
dc.identifier.doi10.1002/advs.202301606
dc.identifier.issn2198-3844
dc.identifier.otherPURE: 75015065
dc.identifier.otherPURE UUID: 7cc8ce2d-39ef-4d53-8aee-51f8ebec4328
dc.identifier.otherScopus: 85170679680
dc.identifier.urihttp://hdl.handle.net/10362/165650
dc.identifier.urlhttps://www.scopus.com/pages/publications/85170679680
dc.language.isoeng
dc.peerreviewedyes
dc.subjectE.coli
dc.subjectFF ATP synthase
dc.subjectgiant vesicles
dc.subjectlipid nanotubes
dc.subjectmicromanipulation
dc.subjectMedicine (miscellaneous)
dc.subjectGeneral Chemical Engineering
dc.subjectGeneral Materials Science
dc.subjectBiochemistry, Genetics and Molecular Biology (miscellaneous)
dc.subjectGeneral Engineering
dc.subjectGeneral Physics and Astronomy
dc.titleRotation of the c-Ring Promotes the Curvature Sorting of Monomeric ATP Synthasesen
dc.typejournal article
degois.publication.titleAdvanced Science
dspace.entity.typePublication
rcaap.rightsopenAccess

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