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Isolation of acute myeloid leukemia blasts from blood using a microfluidic device

2024Artigo científico Acesso aberto
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dc.contributor.authorTeixeira, Alexandra
dc.contributor.authorSousa-Silva, Maria
dc.contributor.authorChícharo, Alexandre
dc.contributor.authorOliveira, Kevin
dc.contributor.authorMoura, André
dc.contributor.authorCarneiro, Adriana
dc.contributor.authorPiairo, Paulina
dc.contributor.authorÁguas, Hugo
dc.contributor.authorSampaio-Marques, Belém
dc.contributor.authorCastro, Isabel
dc.contributor.authorMariz, José
dc.contributor.authorLudovico, Paula
dc.contributor.authorAbalde-Cela, Sara
dc.contributor.authorDiéguez, Lorena
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.institutionCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
dc.contributor.institutionUNINOVA-Instituto de Desenvolvimento de Novas Tecnologias
dc.contributor.pblRSC - Royal Society of Chemistry
dc.date.accessioned2024-09-18T22:20:58Z
dc.date.available2024-09-18T22:20:58Z
dc.date.issued2024
dc.descriptionproject NORTE-01-0145-FEDER-000055, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This project also received funding of the project health from Portugal (C630926586-00465198), supported by Component C5 – Capitalisation and Business Innovation, under the Portuguese Resilience and Recovery Plan, through the NextGenerationEU Fund. B. S.-M. acknowledges funding by FCT, grant number DL 57/2016. The authors kindly thank Dr Oleksandr Bondarchuk for his support in the XPS analysis. This work was carried out in part through the use of the INL User Facilities. Publisher Copyright: © 2024 The Royal Society of Chemistry.
dc.description.abstractAcute myeloid leukemia (AML) is the most common form of acute leukemia in adults and associated with poor prognosis. Unfortunately, most of the patients that achieve clinical complete remission after the treatment will ultimately relapse due to the persistence of minimal residual disease (MRD), that is not measurable using conventional technologies in the clinic. Microfluidics is a potential tool to improve the diagnosis by providing early detection of MRD. Herein, different designs of microfluidic devices were developed to promote lateral and vertical mixing of cells in microchannels to increase the contact area of the cells of interest with the inner surface of the device. Possible interactions between the cells and the surface were studied using fluid simulations. For the isolation of leukemic blasts, a positive selection strategy was used, targeting the cells of interest using a panel of specific biomarkers expressed in immature and aberrant blasts. Finally, once the optimisation was complete, the best conditions were used to process patient samples for downstream analysis and benchmarking, including phenotypic and genetic characterisation. The potential of these microfluidic devices to isolate and detect AML blasts may be exploited for the monitoring of AML patients at different stages of the disease.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent15
dc.format.extent7692494
dc.identifier.doi10.1039/d4an00158c
dc.identifier.issn0003-2654
dc.identifier.otherPURE: 89830300
dc.identifier.otherPURE UUID: 806e8ddf-b69e-4b2f-a6d1-401e9adc7a91
dc.identifier.otherScopus: 85190991458
dc.identifier.otherWOS: 001205994400001
dc.identifier.otherPubMed: 38644740
dc.identifier.otherORCID: /0000-0001-7350-649X/work/167796143
dc.identifier.urihttp://hdl.handle.net/10362/172030
dc.identifier.urlhttps://www.scopus.com/pages/publications/85190991458
dc.language.isoeng
dc.peerreviewedyes
dc.relationinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FEMD-EMD%2F30782%2F2017/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50026%2F2020/PT
dc.relationLA - ICVS/3B's - Associate Laboratory
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationInstitute of Nanostructures, Nanomodelling and Nanofabrication
dc.relationIntegrated Microfluidic Device for cancer detection from free circulating DNA using Digital PCR
dc.relationIntroducing molecular analysis of Circulating Tumour Cells in the clinic: Their significance in clonal evolution and immune escape in gastrointestinal cancers
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F148091%2F2019/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/PDQI/2021.05593.BD/PT
dc.subjectAnalytical Chemistry
dc.subjectBiochemistry
dc.subjectEnvironmental Chemistry
dc.subjectSpectroscopy
dc.subjectElectrochemistry
dc.subjectSDG 3 - Good Health and Well-being
dc.titleIsolation of acute myeloid leukemia blasts from blood using a microfluidic deviceen
dc.typejournal article
degois.publication.firstPage2812
degois.publication.issue10
degois.publication.lastPage2825
degois.publication.titleAnalyst
degois.publication.volume149
dspace.entity.typePublication
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oaire.awardTitleLA - ICVS/3B's - Associate Laboratory
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleInstitute of Nanostructures, Nanomodelling and Nanofabrication
oaire.awardTitleIntegrated Microfluidic Device for cancer detection from free circulating DNA using Digital PCR
oaire.awardTitleIntroducing molecular analysis of Circulating Tumour Cells in the clinic: Their significance in clonal evolution and immune escape in gastrointestinal cancers
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oaire.fundingStream9471 - RIDTI
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso de avaliação no âmbito do Programa Plurianual de Financiamento de Unidades de I&D (2017/2018) - Financiamento Programático
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamConcurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017
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