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Assessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophages

2023-08Artigo científico Acesso aberto
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dc.contributor.authorVentura, Célia
dc.contributor.authorPinto, Fátima
dc.contributor.authorLourenço, Ana Filipa
dc.contributor.authorPedrosa, Jorge F.S.
dc.contributor.authorFernandes, Susete N.
dc.contributor.authorda Rosa, Rafaela R.
dc.contributor.authorGodinho, Maria Helena
dc.contributor.authorFerreira, Paulo J.T.
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.contributor.institutionCentre for Toxicogenomics and Human Health (ToxOmics)
dc.contributor.institutionNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
dc.contributor.institutionDCM - Departamento de Ciência dos Materiais
dc.contributor.institutionCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
dc.contributor.institutionFaculdade de Ciências e Tecnologia (FCT)
dc.contributor.pblMDPI AG
dc.date.accessioned2023-09-13T22:18:27Z
dc.date.available2023-09-13T22:18:27Z
dc.date.issued2023-08
dc.descriptionFunding Information: The work was funded by the Portuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017). The authors also acknowledge the support of FCT/MCTES to the following projects: LA/P/0037/2020, UIDP/50025/2020 and UIDB/50025/2020 of the Associate Laboratory Institute of Nanostructures, Nanomodelling and Nanofabrication-i3N, CIEPQPF (UIDB/00102/2020) and ToxOmics, NMS-UNL (UIDP/00009/2020; UIDB/00009/2020). Thanks also to European Cooperation in Science & Technology (COST) Action: CA21159—Understanding interaction light—biological surfaces: possibility for new electronic materials and devices (PhoBioS); Inpactus Project—innovative products and technologies from eucalyptus (Project No. 21874 funded by Portugal 2020 through ERDF in the frame of COMPETE 2020 No. 246/AXIS II/2017). Publisher Copyright: © 2023 by the authors.
dc.description.abstractCellulose micro/nanomaterials (CMNMs) are innovative materials with a wide spectrum of industrial and biomedical applications. Although cellulose has been recognized as a safe material, the unique properties of its nanosized forms have raised concerns about their safety for human health. Genotoxicity is an endpoint that must be assessed to ensure that no carcinogenic risks are associated with exposure to nanomaterials. In this study, we evaluated the genotoxicity of two types of cellulose micro/nanofibrils (CMF and CNF) and one sample of cellulose nanocrystals (CNC), obtained from industrial bleached Eucalyptus globulus kraft pulp. For that, we exposed co-cultures of human alveolar epithelial A549 cells and THP-1 monocyte-derived macrophages to a concentration range of each CMNM and used the micronucleus (MN) and comet assays. Our results showed that only the lowest concentrations of the CMF sample were able to induce DNA strand breaks (FPG-comet assay). However, none of the three CMNMs produced significant chromosomal alterations (MN assay). These findings, together with results from previous in vitro studies using monocultures of A549 cells, indicate that the tested CNF and CNC are not genotoxic under the conditions tested, while the CMF display a low genotoxic potential.en
dc.description.versionpublishersversion
dc.description.versionpublished
dc.format.extent1395792
dc.identifier.doi10.3390/bioengineering10080986
dc.identifier.otherPURE: 71088564
dc.identifier.otherPURE UUID: 5b7d5878-650b-4cc7-bcef-72497fa413a2
dc.identifier.otherScopus: 85169121029
dc.identifier.otherORCID: /0000-0002-7871-6717/work/151426892
dc.identifier.otherORCID: /0000-0003-1767-3511/work/151431833
dc.identifier.urihttp://hdl.handle.net/10362/157764
dc.identifier.urlhttps://www.scopus.com/pages/publications/85169121029
dc.language.isoeng
dc.peerreviewedyes
dc.subjectbiocompatibility
dc.subjectnanocrystalline cellulose
dc.subjectnanofibrillated cellulose
dc.subjectrespiratory effects
dc.subjectsafety assessment
dc.subjectBioengineering
dc.subjectSDG 3 - Good Health and Well-being
dc.titleAssessing the Genotoxicity of Cellulose Nanomaterials in a Co-Culture of Human Lung Epithelial Cells and Monocyte-Derived Macrophagesen
dc.typejournal article
degois.publication.issue8
degois.publication.titleBioengineering
degois.publication.volume10
dspace.entity.typePublication
rcaap.rightsopenAccess

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